| 张姝 徐凌 李玲香 崔晓波 孙源昊.喉癌中差异表达的蛋白激酶及其抑制剂的生物信息学筛选[J].,2015,15(34):6770-6773 |
| 喉癌中差异表达的蛋白激酶及其抑制剂的生物信息学筛选 |
| Bioinformatics Screening of Differentially Expressed Protein Kinases andtheir Inhibitors in Laryngeal Cancer |
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| DOI： |
| 中文关键词: 喉癌 蛋白激酶 激酶抑制剂 生物信息学 |
| 英文关键词: Laryngeal cancer Protein kinase Kinase inhibitor Bioinformatics |
| 基金项目:内蒙古自治区自然科学基金项目（2014MS0856）；内蒙古医科大学科技百万工程项目（KJbw2013014） |
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| 中文摘要: |
| 目的：筛选喉癌中差异表达的激酶基因以及调控这些差异表达激酶的激酶抑制剂，为喉癌的分子治疗提供新的靶点。方法：
利用PubMed 数据库和SAM 软件筛选喉癌中差异表达的激酶基因。体外培养人喉癌Hep-2和FaDu 细胞。实时定量聚合酶链反
应（Real-time PCR）检测目的激酶在喉癌细胞系中的表达，以验证全基因表达谱结果的准确性。利用KEGG数据库获得激酶调控
的通路。文献挖掘和人工筛选获得差异表达激酶的激酶抑制剂。结果：①在喉癌基因组表达谱中，共筛选出差异表达基因207个，
P<0.05，其中激酶基因4 个，分别为CDC42、CDK7、SLK 和TXK。②Real-time PCR 结果显示在人喉癌Hep-2 和FaDu 细胞中这四
个差异基因也出现差异表达，P<0.05，证明全基因组的结果准确。③通路分析的结果显示4 个差异激酶共调控25 个通路。④文献
挖掘和人工筛选的结果显示共有10 个激酶抑制剂调控CDC42，7 个激酶抑制剂调控CDK7，1 个激酶抑制剂调控SLK，2 个激酶
抑制剂调控TXK。并且再次文献挖掘的结果显示在这20 个激酶抑制剂中，有9 个在癌症方面的研究较少，文献<10 篇。结论：
喉癌中共有四个激酶CDC42、CDK7、SLK 和TXK发生差异表达，并发挥促癌作用。它们的激酶抑制剂可能有潜在的抗癌作用，
为喉癌的分子治疗提供新的切入点。 |
| 英文摘要: |
| Objective:Screening differentially expressed protein kinases and their inhibitors to provide new targets for molecular
therapy of laryngeal.Methods:Pubmed database and SAMsoftware were employed to screen the differentially expressed protein kinase
gene in larybgeal cancer. Hep-2 and FaDu cells were cultured in vitro. Real-time PCR was used to prove the accuracy of microarray
results. Based on KEGG database, these kinase regulated pathways were identified. The kinase inhibitors of differentially expression were
identified by literature mining and artificial screening.Results:①207 differentially expressed genes including 4 protein kinases (CDC42,
CDK7, SLK, TXK) were identified in whole genome profiling(P<0.05). ②The results of Real-time PCR showed that 4 kinase genes were
differentially expressed in Hep-2 and FaDu cells, which consistent with the microarray results(P<0.05). ③The results of pathway analysis
showed 25 pathways were regulated by the 4 kinases. ④The results of literature mining and artificial screening showed that 10 inhibitors
regulated CDC42; 7 inhibitors regulated CDK7; 1 inhibitor regulated SLK and 2 inhibitors regulared TXK. Among them, 9 inhibitors
have few studies in cancer terms (n<10).Conclusion:4 differentially expressed protein kinases (CDC42, CDK7, SLK, TXK) were
identified. Their kinase inhibitors may potential have a role in anti-cancer, which provided a new target point for molecular therapy of
laryngeal cancer. |
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