| 张顺霞 李淑霞 王江 陆勤 姜韬.人桥本甲状腺炎甲状腺组织中miRNAs表达谱的差异性分析[J].,2015,15(36):7171-7174 |
| 人桥本甲状腺炎甲状腺组织中miRNAs表达谱的差异性分析 |
| People Hashimoto Thyroiditis miRNAs in Thyroid Organization Express theDifferences of Spectral Analysis |
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| DOI： |
| 中文关键词: 桥本甲状腺炎 microRNA芯片 差异性 |
| 英文关键词: Hashimoto's thyroiditis(HT) MicroRNA Chip Difference |
| 基金项目:宁夏自然科学基金项目（NZ13038） |
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| 中文摘要: |
| 目的：桥本甲状腺炎是一种自身免疫性甲状腺疾病，且其发病率呈逐年上升趋势，在医疗实践中，HT 被认为是原发性甲状腺
功能减退最常见的原因，同时较易发生甲状腺癌和淋巴瘤。另外，HT 缺乏早期诊断标准，临床上发病隐匿且表现多样，病人多不
易察觉而延误治疗。本文旨在应用microRNA 芯片技术系统筛查HT病变甲状腺组织，以此研究并揭示其HT 的miRNA 表达谱
变化。方法：本研究首先采用microRNA芯片技术，对正常甲状腺组织及桥本甲状腺炎甲状腺组织中microRNA 的表达进行比较，
筛选桥本甲状腺炎中差异性表达的miRNAs。结果：与正常甲状腺相比，在桥本甲状腺炎及其合并甲状腺乳头状癌的一侧桥本甲
状腺炎中分别有39 个和25 个miRNAs 分子发生了差异性表达（P<0.05），比较2 组中共有的miRNAs 发现，miR-142-3p、
miR-338-3p、miR-454、miR-146a、miR-29b-1*、miR-150、miR-223 表达上调，miR-654-5p、miR-601、miR-198、miR-1226* 表达下调
（log2 FC≥ 2，P＜0.05）；而miR-142-5p 在原发性桥本甲状腺炎中表达显著性升高近8 倍（log2 FC=7.959，P＜0.01）。结论：我们通
过microRNA芯片，首次直接系统筛查了桥本甲状腺炎病变组织相关miRNA 表达谱，总体上初步掌握了与正常甲状腺相比，桥
本甲状腺炎及合并甲状腺乳头状癌时其miRNA 表达谱的变化情况，为我们后续的研究提供了方向与基础。 |
| 英文摘要: |
| Objective:Hashimoto thyroiditis is a kind of autoimmune thyroid disease, and there is an upward trend in the incidence
of this disease. In medical practice, HT is believed to be the most common cause of primary hypothyroidism and is considered to be
related to development of both thyroid carcinoma and lymphoma. Because of the lack of early diagnostic criteria of HT, and its diversity
and performance is too difficult to detect, the patient always suffer more delay treatment with poor outcomes. This paper aims to screen
the thyroid tissue of HT lesions and reveal the changes of miRNA expressions of HT by microRNA microarray technology system.Methods:With miRNA array, we compared miRNA expressions of different normal thyroid tissue with HT.Results:The results of
miRNA array showed that, compared with normal thyroid, there had been 39 and 25 miRNAs different expression in HT and HT side of
HT association with papillary thyroid carcinoma （PTC）, respectively (P < 0.05); miR-142-3p, miR-338-3p, miR-454, miR-146a,
miR-29b-1*, miR-150 and miR-223 got the same miRNAs up-regulation（log2 FC≥ 2，P＜0.05）. while miR-654-5p, miR-601, miR-198
and miR-1226*（log2 FC≥ 2, P＜0.05）got down-regulation. In addition，miR-142-5p increased nearly eight times higher in primary HT
than the normal thyroid （log2 FC =7.959, P＜0.01）Conclusion:With the method of microRNA microarray, we found the related
miRNA expression profiles of the Hashimoto's thyroiditis, general initially mastered and compared the miRNA expression profiles of
Hashimoto's thyroiditis with normal thyroid, which laid a foundation for the further functional research of the disease-associated
miRNAs. |
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