| 王国宏 林雪蛟 张磊 龚彤 崔勇华.静磁场对小鼠黑色素瘤细胞B16生长和氧化应激的影响[J].,2016,16(1):76-80 |
| 静磁场对小鼠黑色素瘤细胞B16生长和氧化应激的影响 |
| The Effects of Static Magnetic Fields on the Growth and Oxidative Stress ofMouse Melanoma Cells B16 |
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| DOI： |
| 中文关键词: 中强度静磁场 肿瘤细胞 细胞周期 氧化应激 |
| 英文关键词: Moderate-intensity static magnetic field Tumor cell Cell cycle Oxidative Stress |
| 基金项目:国家自然科学基金项目（31172089） |
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| 中文摘要: |
| 目的：利用小鼠黑色素瘤细胞B16，研究静磁场对肿瘤细胞生长和氧化应激的影响，探讨氧化应激介导静磁场影响肿瘤细胞
生长的机制，为磁场在肿瘤疾病的治疗中的应用提供理论依据。方法：采用MTT 法测定磁场对B16 细胞活力的影响；利用流式细
胞仪测定静磁场暴露对B16 细胞周期分布的影响；利用生物化学方法测定磁场暴露对细胞氧化防御系统相关蛋白酶活性的影
响。结果：24 h内50 mT-200 mT 静磁场暴露可以抑制B16生长，但超过24 h的磁场暴露可以促进B16 生长；100 mT 和200 mT
静磁场暴露对B16的细胞周期分布没有影响；B16 暴露于100 mT 和200 mT 静磁场48 h，GST 活性和GSH/GSSG 水平表现为先
上升后下降，SOD 活性和T-AOC 水平先下降后上升，CAT 活性没有受到影响。结论：50 mT-200 mT 静磁场可以抑制小鼠黑色素
瘤细胞B16 的生长，诱导肿瘤细胞产生氧化应激。 |
| 英文摘要: |
| Objective:The purpose of this research is to investigate the effects of exposing to static magnetic fields (SMFs) on the
growth and oxidative stress of mouse melanoma cells Bl6, and explore the relationship between oxidative stress and the tumor cell
growth, and finally provide a theoretical basis for the application of SMFs in the treatment of tumor disease.Methods:The effects of
SMFs on cells viability were examined by methyl-thiazol-tetrazolium (MTT); the effects of SMFs on cell cycle distribution were detected
by flow cytometry; the effects of SMFs on enzymes activity and antioxidant levels related to antioxidant defense system were monitored
by biochemical methods.Results:Less than 24 hours exposing to SMFs (50 mT-200 mT) could inhibit the growth of B16 , but more than
24 hours exposing to SMFs (50 mT-200 mT) could promote the growth of B16; there's no effects on cell cycle distribution after exposing
to SMFs (100 mT, 200 mT). During 48 hours SMF (100 mT, 200 mT) exposure, a rising trend displayed in the GST activity and the
GSH/GSSG level, and then following a downward trend, at the same time the SOD activity and the total antioxidant capacity displayed a
downward trend and then following a rising trend, and the CAT activity did not change.Conclusion:SMFs (50 mT-200 mT) could inhibit
the growth of B16 cells, and induce oxidative pressure in the B16 cells. |
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