| 秦伟 陈恔 魏怡真 郭丹 金晔△.RNA干扰肝细胞肝癌中PECAM-1的表达对肿瘤细胞侵袭及增殖的影响[J].,2016,16(6):1001-1004 |
| RNA干扰肝细胞肝癌中PECAM-1的表达对肿瘤细胞侵袭及增殖的影响 |
| Effects of PECAM-1 siRNA on the Invasion and Proliferation ofHepatocellular Carcinoma Cells |
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| DOI： |
| 中文关键词: PECAM-1/ CD31 肝细胞肝癌 侵袭 增殖 RNA 干扰 |
| 英文关键词: PECAM-1/ CD31 Hepatocellular carcinoma Migration Proliferation siRNA |
| 基金项目:国家自然科学基金项目（81301845） |
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| 中文摘要: |
| 目的：探讨PECAM-1 在肝细胞肝癌（Hepatocellular carcinoma，HCC）组织中的表达及意义。方法：选择2013 年5 月-2015 年
6 月在我院接受治疗的HCC 患者100 例，收集肝癌患者HCC 组织及癌旁组织，另选取100 例正常肝脏组织作为对照组。应用免
疫组织化学法检测PECAM-1 在肝癌组织、癌旁组织以及正常肝脏组织中的阳性表达。利用小分子干扰RNA 技术（siRNA）构建
低表达的PECAM-1，并转染至肝癌细胞中抑制PECAM-1 的表达。应用Transwell小室法检测肝癌细胞的侵袭能力，CCK-8法检
测肝癌细胞的增殖能力。结果：PECAM-1 在肝癌组织、癌旁组织及正常肝脏组织中呈不同程度阳性表达（P<0.05）；PECAM-1 在肝
癌组织及癌旁组织中的表达显著高于正常肝脏组织，差异具有统计学意义（P<0.05）；PECAM-1 在肝癌组织中的表达显著高于癌
旁组织，差异具有统计学意义（P<0.05）；转染siRNA PECAM-1 后，肝癌细胞中PECAM-1 mRNA的表达水平明显下降，PECAM-1
蛋白表达也明显降低，差异具有统计学意义（P<0.05）；转染siRNA PECAM-1 后，肝癌细胞侵袭及增殖能力明显降低，差异具有统
计学意义（P<0.001）。结论：PECAM-1 在肝癌患者血清中高表达，PECAM-1 siRNA 能够抑制肝癌细胞的侵袭及增殖能力，提示
PECAM-1 可作为预测肝癌发生及发展的临床指标。 |
| 英文摘要: |
| Objective:To investigate the expression and significance of PECAM-1 in hepatocellular carcinoma (HCC).Methods:100 patients with hepatocellular carcinoma who had taken the resection surgery in our hospital from May 2013 to June 2015 were
selected, and the HCC cancer tissues and the para-carcinoma tissues were collected. Another 100 normal liver tissues were collected as
the control group. Then the expressions of PECAM-1 in the tumor tissues, the para-carcinoma tissues and the normal liver tissues were
detected by RT-PCR. The small interfering RNA (siRNA) was used to inhibit the expression of PECAM-1 in hepatocellular carcinoma.
The invasion of hepatocellular carcinoma cells was detected by Transwell, and the proliferation of hepatocellular carcinoma cells was
detected by CCK-8.Results:PECAM-1 positively expressed in HCC tissues, adjacent tissues and normal liver tissues (P<0.05). The
expressions of PECAM-1 in HCC tissues and adjacent tissues were significantly higher than that of the normal liver tissues (P<0.05). The
expression of PECAM-1 in HCC tissues was significantly higher than that of the adjacent tissues (P<0.05). The level of PECAM-1
significantly decreased after transferred by PECAM-1 siRNA, and the invasion and proliferation of HCC cells significantly decreased,
and the differences were statistically significant (P<0.05).Conclusion:PECAM-1 highly expressed in serum of patients with liver
cancer, and siRNA PECAM-1 can inhibit the invasion and proliferation of HCC cells, that PECAM-1 can be used as a clinical indicator
for predicting the occurrence and development of liver cancer. |
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