| 杨欣 李熙鸿△ 苏云洁 李亚飞 刘忠强.氯化锂对脓毒症大鼠血清促炎细胞因子及氧化损伤的影响[J].,2016,16(6):1005-1007 |
| 氯化锂对脓毒症大鼠血清促炎细胞因子及氧化损伤的影响 |
| Effects of LithiumChloride on SerumProinflammatory Factor and OxidativeDamage in Septic Rats |
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| DOI： |
| 中文关键词: 氯化锂 脓毒症 促炎细胞因子 氧化损伤 |
| 英文关键词: LithiumChloride Sepsis Proinflammatory factor Oxidative damage |
| 基金项目:国家自然科学基金项目(30500544)；教育部长江学者和创新团队发展计划（IRT09350）；四川省科技厅支撑项目（12ZCO909）
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| 中文摘要: |
| 目的：探讨氯化锂对脓毒症大鼠的促炎细胞因子及氧化损伤的影响。方法：将50只Wistar 大鼠按随机数字表随机分为假手
术组（S组，n=10）、模型组（CLP 组，n=20）、氯化锂干预组（CLP+LiCl 组，n=20），每组又分为8 h、16 h、24 h 3 个时间观察点亚组，
CLP组采用盲肠结扎穿孔术（CLP）制备脓毒症模型。氯化锂干预组于CLP 手术后立即给予腹腔注射LiCl溶液（50 mg/Kg），假手
术组和CLP 组给予等量生理盐水腹腔注射。每组每只大鼠分别于术后8 h、16 h、24 h采集眼眶静脉血检测其中肿瘤坏死因子-alpha
（TNF-alpha）、白介素6（IL-6）、超氧化物歧化酶（SOD）、丙二醛（MDA）浓度，并记录术后24 h各组大鼠生存率。结果：与S组相比，
CLP 组及CLP+LiCl 组各时间点血清TNF-alpha、IL-6 和MDA 的浓度上升，SOD浓度降低，差异有统计学意义（P＜ 0.05）；与CLP 组
同时间点比较，CLP+LiCl 组血清TNF-琢、IL-6 和MDA的浓度下降，SOD浓度升高；与CLP 组术后24h 生存率相比，CLP+LiCl 组
生存率提高，差异有统计学意义（P＜ 0.05）。结论：氯化锂可能通过抑制炎性细胞因子释放以及减轻氧化应激损伤的机制实现提高
脓毒症大鼠生存率的作用。 |
| 英文摘要: |
| Objective:To investigate the effects of Lithium Chloride on serum Proinflammatory factor and oxidative damage in
septic rats.Methods:Fifty male rats were randomLy divided into shamoperated group（S group, n＝10）, model group（CLP group, n＝
20）, and Lithium chloride treated group （CLP+LiCl group, n＝20）. The living rats in each group were divided into three subgroups
according to time of getting sample (8, 16, 24 h). Models of rats with sepsis were established by cecal ligation and puncture (CLP). In
CLP+LiCl group, LiCl solution（50 mg/Kg）was injected into the rats peritoneal cavity after the operation of CLP immediately. Orbital
venous blood samples were taken from each rat in these groups at 8, 16, and 24 hours after CLP, then the levels of TNF-alpha, IL-6, SOD,
MDA were measured, and each group's survival rate were recorded within 24 hours after CLP.Results:Compared with S group, The
levels of TNF- 琢, IL-6, MDA at that three time points were increased in both CLP group and CLP+LiCl group, and the level of SOD was
decreased at the same time. There were statistical significant differences between them (P＜0.05); Compared with CLP group, The levels of
TNF-琢, IL-6, MDA were decreased in CLP+LiCl group, and the level of SOD was increased at the same time. There were statistical
significant differences between them (P＜0.05). Compared with CLP group, the survival rate of CLP+LiCl group was increased at 24 hours
after the operation of CLP, and there were statistical significant differences between them (P＜0.05).Conclusion:Lithium Chloride can
increase the survival rate of septic rats may through the mechanism of inhabiting the release of Proinflammatory cytokines and relieving
oxidative damage. |
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