| 石子璇 李军昌 李哲 谢娟 刘倩 杜志超 曹亮 张莎 王宗仁.模拟微重力对恶性胶质瘤细胞U87 影响的实验研究[J].,2016,16(8):1411-1414 |
| 模拟微重力对恶性胶质瘤细胞U87 影响的实验研究 |
| Experimental Study of Effect of Modeled Microgravity on MalignantGlioblastoma Cell U87 |
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| DOI： |
| 中文关键词: 模拟微重力 胶质瘤细胞 增殖 基质金属蛋白酶 恶性表型 |
| 英文关键词: Modeled microgravity Glioma Proliferation MMPs Malignant phenotype |
| 基金项目:国家自然科学基金面上项目（81273879） |
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| 中文摘要: |
| 目的：探讨模拟微重力（Modeled microgravity，MMG）对恶性胶质瘤细胞U87 形态、生长增殖、基质金属蛋白酶-2、-9（Matrix
metalloproteinase 2，MMP-2、Matrix metalloproteinase 9，MMP-9）及神经胶质酸性蛋白（Glial fibrillary acidic protein，GFAP）表达的
影响。方法：常规培养U87细胞，传代培养3 代后分为两组，正常重力组（Normal gravity，NG组）及模拟微重力干预组（Modeled
microgravity，MMG 组），相应条件下培养24 h，48 h和72 h。倒置显微镜观察U87 细胞形态改变，细胞计数法及四甲基偶氮唑盐
微量酶反应比色法（3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide，MTT 法）检测模拟微重力干预和干预后U87 细胞
生长增殖情况；Western Blot 检测不同培养时间后胶质瘤细胞U87 MMP-2、MMP-9 及GFAP 蛋白的表达情况。结果：模拟微重
力条件下培养72 h后，U87细胞轮廓不规则，边缘圆钝，触角变少；模拟微重力条件下，U87 细胞生长速度明显减慢，并且经模拟
微重力干预48 h和72 h后的胶质瘤细胞经传代再培养，其增殖率也明显低于正常重力组；同时，U87细胞MMP-2 及MMP-9 蛋
白表达水平与模拟微重力处理时间呈时间依赖性下降，而GFAP蛋白表达水平则呈时间依赖性升高。结论：模拟微重力影响
U87 细胞的形态、生长及表型。 |
| 英文摘要: |
| Objective:To investigate the effect of modeled microgravity on morphology, proliferation and the protein expression
of Matrix metalloproteinase 2(MMP-2), Matrix metalloproteinase 9（MMP-9）and Glial fibrillary acidic protein（GFAP）of a glioma cell
line U87.Methods:After cultured for 3 passages, U87 cells were divided into two groups: normal gravity group (NG) and modeled
microgravity group (MMG), and cultured for 24 h, 48 h and 72 h in corresponding conditions. The changes of morphology were observed
by inverted microscope; the cytometry assay and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide（MTT）assay were used
to evaluate the growth rate of the U87 cells under or after MMG treatment; Western Blot was applied to analyze the MMP-2, MMP-9 and
GFAP protein expression of the U87 cells.Results:After cultured for 72 h under MMG condition, the morphology of U87 cells changed
into being irregular and turned to be acuity; under MMG condition, the growth rate of the U87 cells was significantly slowed down,
especially for 48 h and 72 h, compared with that of U87 cells under NG condition. Furthermore, after cultured under MMG condition for
48 h and 72 h not for 24 h, the sub-cultured U87 cells grew more slowly under NG condition, compared those of NG group; Additionally,
the expression of MMP-2 and MMP-9 of U87 cells was reduced in time-dependent after exposed to MMG. Nevertheless, the expression
of GFAP was up-regulated under MMG condition.Conclusion:MMG could change the morphology, proliferation and phenotype of U87
cells. |
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