| 孙鸣宇 闫承慧 田孝祥 李洋 韩雅玲.CREG促进小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达*[J].,2016,16(8):1424-1427 |
| CREG促进小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达* |
| CREG Promotes Lysosome Biogenesis and Expression of Cathepsins inMouse Peritoneal Cavity Macrophages |
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| DOI： |
| 中文关键词: E1A激活基因阻遏子 巨噬细胞 溶酶体 |
| 英文关键词: Cellular repressor E1A-stimulated genes (CREG) Macrophages Lysosome |
| 基金项目:国家自然科学基金项目（81130072, 81370243） |
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| 中文摘要: |
| 目的：研究E1A激活基因阻遏子（Cellular repressor of E1A-stimulated genes，CREG）对小鼠腹腔巨噬细胞溶酶体发生及溶酶
体组织蛋白酶表达的影响。方法：应用loss-of-function 和gain-of-function 模型，Lysotracker Red 染色和透射电镜观察CREG对小
鼠腹腔巨噬细胞溶酶体发生的影响，并通过细胞免疫荧光染色和Western blot 方法，观察CREG对小鼠腹腔巨噬细胞溶酶体组织
蛋白酶表达的影响。结果：Lysotracker Red 染色和透射电镜证实CREG可促进小鼠腹腔巨噬细胞溶酶体发生；细胞免疫荧光染色
和Western blot方法证实CREG可促进小鼠腹腔巨噬细胞溶酶体组织蛋白酶cathepsin B及cathepsin S 表达。结论：CREG 可促进
小鼠腹腔巨噬细胞溶酶体发生及组织蛋白酶cathepsin B及cathepsin S 表达。 |
| 英文摘要: |
| Objective:To study the effects of cellular repressor of E1A stimulated genes (CREG) on lysosome biogenesis and
expression of cathepsins in mouse peritoneal cavity macrophages.Methods:Lysotracker Red staining and transmission electron
microscope were applied to observe the effect of CREG on lysosome biogenesis in mouse peritoneal cavity macrophages using
gain-of-function and loss-of-function approaches. The effect of CREG on expression of cathepsins in mouse peritoneal cavity
macrophages were studied by immunofluorescence staining and Western blot.Results:It was confirmed that CREG can promote
lysosome biogenesis in mouse peritoneal cavity macrophages using Lysotracker Red staining and transmission electron microscope. The
findings of immunofluorescence staining and Western blot provided evidence that CREG promoted expression of cathepsin B and
cathepsin S in mouse peritoneal cavity macrophages.Conclusion:CREG promotes lysosome biogenesis and expression of cathepsin B
and cathepsin S in mouse peritoneal cavity macrophages. |
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