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朱紘萱廖前德黄建军尹科钟达.Wnt5a、Ror2在骨肉瘤中的表达及临床意义[J].,2016,16(8):1467-1471

Wnt5a、Ror2在骨肉瘤中的表达及临床意义

Expression and Clinical Significance of Wnt5a and Ror2 in Osteosarcoma

DOI：

中文关键词: 骨肉瘤 Wnt5a Ror2 免疫组织化学生物学行为

英文关键词: Osteosarcoma Wn5a Ror2 Immunohistochemistry Biology behavior

基金项目:湖南省医药卫生科研计划课题项目（B2013-160）

作者	单位
朱紘萱廖前德黄建军尹科钟达	中南大学湘雅医院骨科；湘西自治州人民医院暨吉首大学第一附属医院骨二科；南华大学附属第一医院

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中文摘要:

目的：探讨骨肉瘤组织中Wnt5a、Ror2 表达。方法：免疫组织化学法检测Wnt5a、Ror2 在实验组55 例骨肉瘤和对照组15 例骨软骨瘤组织标本中的表达。结果：Wnt5a、Ror2 在骨肉瘤组的表达均明显高于骨软骨瘤组（Wnt5a：74.55 %、20.00 %，Ror2：13.33 %、70.91 %，P<0.05）。Wnt5a、Ror2 在骨肉瘤出现转移的表达明显高于未转移（Wnt5a：100 %、66.67 %，Ror2：100 %、61.90 %，P<0. 05），在骨肉瘤Enneking 分期中，Wnt5aⅠ期11.11 %，Ⅱ期84.38 %，Ⅲ期100.00 %，Ror2Ⅰ期22.22 %，Ⅱ期72.73 %，Ⅲ期100.00 %，Ⅰ期与Ⅱ期间和Ⅰ期与Ⅲ期间差异皆有统计学意义（P<0.05），而Ⅱ 期与Ⅲ 期间差异皆无统计学意义（P>0.05），Wnt5a、Ror2 两者之间的表达呈正相关分布（骨肉瘤组：r=0.844，P<0.01；骨软骨瘤组：r=0.808，P<0.01），但在骨肉瘤患者性别、年龄以及骨肉瘤病理分型组中表达无明显差异（P>0.05）。结论：Wnt5a、Ror2 在骨肉瘤中存在高度表达，可能具有促癌作用，并与其恶性程度和侵袭转移有关，二者协同作用。

英文摘要:

Objective:To investigate the expression and clinical significance of Wnt5a and Ror2 in human osteosarcoma tissues.Methods:SP immunohistochemistry was performed to detect the expressions of Wnt5a and Ror2 protein in specimens of 55 osteosarcoma patients and 15 osteochondroma patients. Then the interrelation of the expressions of wnt5a and Ror2 were calculated and analyzed.Results:The positive rate of both Wnt5a and Ror2 expression showed significant differences between osteosarcoma group and osteochondroma group (Wnt5a: 74.55%, 20.00%, Ror2: 13.33%, 70.91%, P<0.05), and showed significant differences between osteosarcoma metastasis group and non-metastasis group (Wnt5a: 100 %, 66.67 %; Ror2: 100 %, 61.90 %, P<0.05). In osteosarcoma Enneking stage, the expression ofWnt5a in I, Ⅱ and Ⅲ stage was11.11%, 84.38 %, Ⅲ 100.00%, respectively; the expression of Ror2 in I, Ⅱ and Ⅲ stage was 22.22 %, Ⅱ 72.73 %, 100.00 %, respectively. The positive rate of Wnt5a was similar to Ror2, and both of them were statistically significant between Enneking stage Ⅰ and Ⅱ, Ⅰ and Ⅲ (P<0.05), but no significance between Enneking stage Ⅱ and Ⅲ (P>0.05). A positive correlation existed between the expressions of wnt5a and Ror2 (osteosarcoma: r=0.844, P<0.01; osteochondroma: r=0.808, P<0.01), but the differences of the positive rate of bothWnt5a and Ror2 in osteosarcoma sex, age and pathological type were not statistically significant (P>0.05).Conclusion:Wnt5a and Ror2 may act as a tumor promoter in osteosarcoma with synergistic effect, which are positively related to the severity, invasion and metastasis of osteosarcoma.

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