| 李沃 黄东 胡霞 黄苏萍 孔高茵 刘际童.三种不同表面活性剂对羟基磷灰石纳米颗粒的表面修饰的比较[J].,2016,16(11):2013-2017 |
| 三种不同表面活性剂对羟基磷灰石纳米颗粒的表面修饰的比较 |
| Surface Modification of Hydroxyapatite Nanoparticle with DifferentSurfactants |
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| DOI： |
| 中文关键词: 纳米复合物 羟基磷灰石 CTAB PEG2000 人血清 表面修饰 |
| 英文关键词: Nano-composites Hydroxyapatite CTAB PEG2000 Human blood serum Surface modification |
| 基金项目:国家自然科学基金项目（81172546）；湖南省自然科学基金项目（13JJ2015） |
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| 中文摘要: |
| 目的：本研究旨在通过不同方法修饰羟基磷灰石纳米颗粒并检测其稳定性及分散性。方法：首先采用水合热合成法制备羟
基磷灰石纳米颗粒，然后用透射电镜（TEM）和场发射扫描电镜（SEM）对其表面形态结构进行表征。我们首次用溴化十六烷三甲
基铵（CTAB），PEG2000 和人血清对羟基磷灰石纳米颗粒通过共价结合或表面吸附的方式进行表面嫁接，并利用透射电镜，傅里
叶红外光谱（FT-IR）和X 射线衍射（XRD）对新合成的这三种纳米羟基磷灰石复合物的形貌，结构和晶粒粒径进行表征。对这三种
羟基磷灰石纳米颗粒悬浮液的时间沉降曲线进行分析。在分散性上通过检测这三种羟基磷灰石复合物悬浮液在不同pH 值下的
Zeta 电位并绘制Zeta-pH 曲线。结果：我们发现CTAB 修饰的羟基磷灰石纳米颗悬浮液的悬浮稳定性最佳，其次是PEG2000，最
后是人血清。在pH=7.0 时，CTAB 修饰的羟基磷灰石纳米颗粒的zeta 电位值是25.68 mV，而PEG2000 修饰的Zeta 电位是4.32
mV，人血清修饰的Zeta 电位是-13.23mV。结论：CTAB表面修饰的羟基磷灰石纳米颗粒相对于其它两种表面活性剂复合物具有
更好的分散性和悬浮稳定性，与DNA/RNA 结合能力更强。本课题的结果给羟基磷灰石纳米颗粒载体的应用提供了一种新的选
择，有望利用亲和力更高的基因载体实现基因治疗，具有广阔的应用前景。 |
| 英文摘要: |
| Objective:We have the hydroxyapatite (HA) nanoparticles surface grafted by three chemicals to find the stability and
their suspension dispensability of these HA Nano-composite aqueous solutions.Methods:Hydroxyapatite (HA) nanoparticles were well
prepared by chemical co-precipitation hydrothermal synthesis and their topography feature characterized by transmission electron microscope
(TEM) and field emission scanning electron microscopy (FE-SEM). Hydroxyapatite (HA) nanoparticles were firstly surface grafted
with Cetyltrimethyl Ammonium Bromide (CTAB), PEG 2000 and human blood serum via covalent binding or surface adsorption. The
structures and properties of the various HA Nano-composites were investigated by transmission electron microscope (TEM), Fourier
transform infra-red spectroscopy (FT-IR) and X ray diffraction (XRD) which confirmed the modification reaction on HA nanoparticles
surfaces, then draw their settlement curves. In order to better understand their suspension dispensability, we detected the zeta potentials of
the three HA nano-composites solution under different pH separately and drew the zeta-pH curve.Results:Comparing the settlement
curve of the three HA nano-composite aqueous solutions, we found that CTAB modified HA nanoparticles had the best suspension stability,
then PEG2000, the last human blood serum. The result of zeta potential of CTAB modified HA is about 25.68 mV at pH of 7.0, while
PEG2000 modified HA is about 4.32 mV and human serum modified HA is -13.23 mV.Conclusion:Compared to other two sufactants,
CTAB modified HA had better dispersibility and suspension stability, and the positive electron CTAB-HA carrier could bind negative
DNA/RNA better The results of this study could provide a new selection during the application of hydroxyapatite nanoparticle vectors in
biomedicine. |
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