| 曹海燕 翟玉龙 孙夏承 安家泽 吕英华 邢金良△ 黄启超.基于电镜和免疫组化的线粒体数目标志物评估肝癌的初步研究[J].,2016,16(14):2646-2650 |
| 基于电镜和免疫组化的线粒体数目标志物评估肝癌的初步研究 |
| Evaluation of Hepatocellular Carcinoma Growth Based on MitochondrialMolecular Marker using Transmission Electron Microscope andImmunohistochemistry |
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| DOI： |
| 中文关键词: 线粒体定量 电子显微镜 COX IV HSP60 |
| 英文关键词: Quantification of mitochondria Transmission electron microscope COX IV HSP60 |
| 基金项目:国家自然科学基金项目（81572410；31401221） |
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| 摘要点击次数: 922 |
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| 中文摘要: |
| 目的：研究线粒体数目与肝癌生长的相关性。方法：利用电子显微镜技术定量肝癌组织中线粒体数目，利用免疫组织化学技
术评估肝癌组织中的线粒体标志物COX IV 及HSP60 表达水平，分析电镜肝癌线粒体定量结果与COX IV 及HSP60 表达量之间
的相关性，并比较肝癌中线粒体数目、COX IV 及HSP60 表达量与肝癌直径之间的关系。结果：与癌旁相比，肝癌组织中线粒体数
目（P＜0.001）、COX IV 及HSP60的表达量显著降低（P=0.0417，P=0.0290）。COX IV 及HSP60 表达水平与线粒体数目无显著相关
（r2=0.009，P=0.5468；r2=0.056，P=0.1396）。线粒体数目与肿瘤直径显著负相关（r2=0.1086，P=0.0434），COX IV 及HSP60表达量与
肿瘤直径无显著相关（r2=0.0251，P=0.3287；r2=0.0461，P=0.1830）。结论：线粒体数目是潜在的肝癌生长标志物。但常用的线粒体定
量分子HSP60 与COX IV 并不能准确定量肝癌线粒体。 |
| 英文摘要: |
| Objective:To evaluate the correlation between mitochondrial number and tumor growth in Hepatocellular carcinoma
(HCC).Methods:Transmission electron microscope was used to quantify the number of mitochondria in HCC tissues.
Immunohistochemistry was used to evaluate the expression levels of COX IV and HSP60 in HCC tissues.Results:Mitochondrial number
(P＜0.001) and the expression levels of COX IV and HSP60 (P=0.0417, P=0.0290) were decreased in HCC tissues when compared to
paired non-tumor tissues. Correlation analysis showed that mitochondrial number (r2=0.1086, P=0.0434), but not COX IV and HSP60
(r2=0.009, P=0.5468; r2=0.056, P=0.1396), was significantly negatively associated with tumor diameter. No significant correlation was
found between the expression level of COX IV or HSP60 and mitochondrial number in HCC tissues (r2=0.0251, P=0.3287; r2=0.0461,
P=0.1830).Conclusion:Mitochondrial number is a potential biomarker for evaluating tumor growth and prognosis of HCC. Whereas,
the commonly used molecular markers COX IV and HSP60 are not suitable for quantification of mitochondria in HCC tissues. |
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