| 张一恺 徐慧 尚沛津 刘天龙 丁一 朱志会 王明明 李玉文 文爱东.alpha- 乳香酸联用羟基红花黄色素A抗胃溃疡效果研究[J].,2016,16(17):3232-3236 |
| alpha- 乳香酸联用羟基红花黄色素A抗胃溃疡效果研究 |
| Gastro-protective Effects of the Combination of alpha-Boswellic Acid and HSYAon Ethanol-induced Gastric Injury in Rats |
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| DOI： |
| 中文关键词: alpha-乳香酸 羟基红花黄色素A 胃溃疡 抗炎 |
| 英文关键词: alpha-Boswellic Acid Hydroxysafflor Yellow A Gastric ulcer Anti-inflammatory |
| 基金项目:国家自然科学基金项目（81373947；81201985） |
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| 中文摘要: |
| 目的：研究乳香红花提取物中主要药效物质alpha-乳香酸（alpha-BA）和羟基红花黄色素A（HSYA）联合应用的胃保护效果。方法：
36 只SD 大鼠随机分为6 组：正常组（生理盐水5 mL/kg），模型组（生理盐水5 mL/kg），西咪替丁组（100 mg/kg），HSYA 组（100
mg/kg），alpha-BA 组（200 mg/kg），联合用药组（HSYA 50 mg/kg+alpha-BA 100 mg/kg）。预给药1 小时后，用乙醇诱导SD 大鼠胃溃疡造
模，评价溃疡指数、胃内容物酸度、胃壁黏液量，用ELISA法检测血清炎性因子TNF-alpha、IL-1beta、IL-8。结果：①与造模组相比，alpha-BA
组和联合用药组显著降低乙醇诱导胃粘膜损伤（P＜0.001）且联合用药组较西咪替丁组相比差异无统计学意义，但HSYA组差异
无统计学意义；与造模组相比，alpha-BA组和联合用药组显著升高胃内容物pH，增加胃黏液含量（P＜0.001）且联合用药组与西咪替
丁组相比差异无统计学意义，但HSYA 组仅胃黏液含量有一定升高（P＜0.05），胃内容物pH 没有明显改变。②与造模组相比，
HSYA 组、alpha-BA组、联合用药组均显著抑制血清TNF-alpha、IL-1beta、IL-8 的升高（P＜0.001）且联合用药组与西咪替丁组相比差异无统
计学意义。③H&E 染色观察可见HSYA组依然有明显出血性损伤；西咪替丁组、琢-BA组和联合用药组则明显保护胃黏膜、抑制
损伤和白细胞浸润。结论：alpha-BA有较好的胃保护效果，HSYA胃保护效果显著低于alpha-BA，但联合应用HSYA、alpha-BA能够抑制胃
黏膜出血、白细胞浸润、抑制胃内容物酸度，增加胃黏液含量，并且能够调节血清炎性因子从而发挥较单用药用量更少且效果更
好的胃保护作用。 |
| 英文摘要: |
| Objective:To investigate the protective effects of alpha-Boswellic Acid and HSYA on ethanol-induced gastric injury in
rats.Methods:All the 36 rats were divided evenly and randomly into the following 6 groups: control group (5 mL/kg physiological
saline), model group (5 mL/kg physiological saline), cimetidine group (100 mg/kg), HSYA group (100 mg/kg), alpha-BA group (200 mg/kg),
combination group (HSYA 50 mg/kg+alpha-BA 100 mg/kg). One hour before administration of absolute ethanol, the vehicle, cimetidine and
various doses of HSYA and alpha-BA were given to each experimental group through administration by gavage. The gastro-protection of effects
was assessed by measuring Ulcer Index (UI), gastric juice acidity (pH), gastric wall mucus (GWM) and the level of serum TNF-alpha,
IL-1 beta and IL-8 by ELISA.Results:① Compared with model group, UI was decreased significantly in 琢-BA group and combination
group (P＜0.001) and the differences between cimetidine group and combination group were not statistical significance, while the difference
between HSYA group and model group were not statistical significance; Compared with model group, and the pH and GWM were
increased markedly in 琢-BA group and combination group (P＜0.001) and the differences between cimetidine group and combination
group were not statistical significance, however, difference between HSYA group and model group were only statistical significance in
GWM (P＜0.05). ② Compared with control group, the level of serum TNF-alpha, IL-1betaand IL-8 were significantly in- creased in HSYA group (P＜0.05), alpha-BA group and combination group (P＜0.001) and the differences between cimetidine group and combination group
were not statistical significance. ③ For H&E staining, cimetidine group, alpha-BA group and combination group protected the gastric tissues
from hemorrhagic lesions along with attenuation of leukocyte infiltration and submucosal edema.Conclusion:The gastroprotective
effects of alpha-BA was better than HSYA, and combination of 琢-BA and HSYA may protect gastric fromhemorrhagic lesions along with attenuation
of leukocyte infiltration and submucosal edema through mediating serum TNF-alpha, IL-1beta, IL-8 and gastric juice acidity and gastric
wall mucus. |
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