| 于英君 韩松洋 姜颖 张梅 杨丹 葛圆圆 于水澜.基于肝癌CDK2 基因敲减对CyclinE 表达及树舌多糖GF对其影响的研究[J].,2016,16(23):4434-4437 |
| 基于肝癌CDK2 基因敲减对CyclinE 表达及树舌多糖GF对其影响的研究 |
| Study on the Effect of CDK2 Knockdown Decreases on the Expression ofCyclinE and GAPS.GF in Hepatocellular Carcinoma |
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| DOI： |
| 中文关键词: 肝癌HepG2 细胞 rAAV-shRNA-CDK2 树舌多糖GF（GAPS.GF） CyclinE |
| 英文关键词: Hepatocellular carcinoma HepG2 cells rAAV-shRNA-CDK2 GAPS.GF CyclinE |
| 基金项目:黑龙江省中医管理局项目（ZHY06-Z02）；黑龙江中医药大学科研基金项目（X201004）；
黑龙江中医药大学科研基金项目（201409）；黑龙江省博士后基金项目（LBH-Z15211） |
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| 中文摘要: |
| 目的：探究树舌多糖GF（GAPS.GF）对rAAV-shRNA-CDK2 抑制肝癌细胞CyclinE 基因表达的辅助作用。方法：将细胞培养
后的人肝癌HepG2 以皮下注射的方式接种于裸鼠前肢腋下，接种后的裸鼠随机分为5组：NC（非相关序列）对照组、肿瘤组、
rAAV-shRNA-CDK2 组、树舌多糖GF组以及树舌多糖GF+rAAV-shRNA-CDK2 组，各实验组均采取尾静脉注射定量给药。采用
实时定量PCR和Western blot 技术研究肝癌细胞CyclinE 基因mRNA和蛋白水平的表达情况，同时观察GASP.GF 对其作用的
影响。结果：树舌多糖GF+rAAV-shRNA-CDK2 联合应用组对肝癌HepG2 细胞增殖的抑瘤率为75.6 %，对CyclinE 基因mRNA
表达抑制率为69 %，对CyclinE 基因蛋白表达抑制率为67.5 %，比rAAV-shRNA-CDK2 组分别提高了3.42 %、1 %和2.7 %。结
论：树舌多糖GF 与rAAV-shRNA-CDK2 联合应用可以显著提高肝癌的治疗效果，说明树舌多糖GF 可以辅助rAAV-shRNACDK2
对肝癌细胞CyclinE 基因的表达的抑制作用。 |
| 英文摘要: |
| Objective:To study the auxiliary function of GAPS.GF on inhibition the CyclinE gene expression in hepatocellular
carcinoma cell by rAAV-shRNA-CDK2.Methods:Human hepatocellular carcinoma HepG2 cells were cultured and inoculated into nude
mice by subcutaneous injection. The nude mice were randomly divided into 5 groups: a NC contrast group, a tumor group, a
rAAV-shRNA-CDK2 group, a GAPS.GF group and a GAPS.GF+ rAAV-shRNA-CDK2 group, all the experimental groups were administered
by caudal vein. Using Real-time PCR and Western blot to study the expression of mRNA and protein levels of CyclinE gene in
hepatocellular carcinoma cells and then observing the effect of GAPS.GF on its function.Results:The inhibition rate of proliferation of
hepatocellular carcinoma HepG2 cells was 75.6 % in the combination group of GAPS.GF+rAAV-shRNA-CDK2 group, the inhibition
rate of CyclinE gene mRNA expression was 69 %, the inhibition rate of CyclinE gene protein expression was 67.5 %, than the
rAAV-shRNA-CDK2 group were increased by 3.42 %, 1 %, 2.7 % .Conclusion:The combined application of GAPS.GF and
rAAV-shRNA-CDK2 can significantly improved the treatment effect of liver cancer, which shows that the GAPS.GF can help
rAAV-shRNA-CDK2 to inhibit the expression of CyclinE gene in hepatocellular carcinoma cells. |
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