| 刘丹 田孝祥 张效林 高乃婧 闫承慧△.趋化因子CCL2对人脐静脉内皮细胞ICAM-1 表达的影响[J].,2016,16(26):5024-5027 |
| 趋化因子CCL2对人脐静脉内皮细胞ICAM-1 表达的影响 |
| Effect of Chemokine CCL2 on the ICAM-1 Expression of HUVECs |
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| DOI： |
| 中文关键词: 趋化因子配体2 人脐静脉内皮细胞 细胞间粘附分子-1 动脉粥样硬化 |
| 英文关键词: C-C motif ligand 2 (CCL2) Human umbilical vein endothelial cells (HUVECs) Intercellular adhesion molecule-1
(ICAM-1) Atherosclerosis |
| 基金项目:国家自然科学基金青年基金项目(81500282) |
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| 中文摘要: |
| 目的：探讨CC类趋化因子配体2 (C-C motif ligand 2，CCL2) 对人脐静脉内皮细胞(human umbilical vein endothelial cells，
HUVECs)中细胞间粘附分子-1(intercellular adhesion molecule-1，ICAM-1)表达的影响。方法：体外分离培养HUVECs 细胞，将
HUVECs 铺至6 孔板中，待细胞融合至80-90 %时，将CCL2 过表达载体[pcDNA3.1(+)-CCL2]及CCL2 小分子干扰RNA(si-RNA)
分别转染到HUVECs 中，于转染后12 h、24 h和48 h 收集细胞进行RNA 及蛋白提取。荧光定量PCR 方法检测HUVECs 中
CCL2及ICAM-1基因mRNA 表达。Western blotting 检测HUVECs中CCL2 及ICAM-1 蛋白表达。结果：(1)与pcDNA3.1(+)组相
比较，pcDNA3.1 (+)-CCL2 组中CCL2 基因mRNA 和蛋白水平均显著升高；与si-Control 组相比较，si-CCL2 组中CCL2 基因
mRNA和蛋白表达均明显下降。(2)与对照组比较，pcDNA3.1(+)-CCL2 组明显增加HUVECs 中ICAM-1 的mRNA 及蛋白表达，而
si-CCL2 组显著抑制HUVECs中ICAM-1 的mRNA及蛋白表达。结论：CCL2 能增加HUVECs 中ICAM-1 基因mRNA 和蛋白表
达，为深入认识动脉粥样硬化的发病机制提供了理论依据。 |
| 英文摘要: |
| Objective:To observe the effect of C-C motif ligand2 (CCL2) on the expression of intercellular adhesion molecule-1
(ICAM-1) in human umbilical vein endothelial cells (HUVECs).Methods:HUVECs cells were isolated and cultured in vitro. HUVECs
were plated into 6-well plate, when cells were grown to 80-90 %, CCL2 overexpression plasmid [pcDNA3.1 (+)-CCL2] or CCL2 small
interfering RNA (si-CCL2) was transfected into HUVECs. At different time points after transfection (12 h, 24 h and 48 h), HUVECs were
collected for RNA and protein extraction. The mRNA expressions of CCL2 and ICAM-1 were examined using quantitative RT-PCR. The
protein expressions of CCL2 and ICAM-1 were examined using western blotting.Results:(1) Compared with pcDNA3.1 (+) group, the
expressions of CCL2 mRNA and protein were significantly higher in pcDNA3.1 (+)-CCL2 group; Compared with si-Control group, the
expressions of CCL2 mRNA and protein were significantly decreased in si-CCL2 group. (2) Compared with pcDNA3.1 (+) group,
pcDNA3.1 (+)-CCL2 significantly increased the mRNA and protein expressions of ICAM-1 in HUVECs, and si-CCL2 significantly
inhibited the mRNA and protein expressions of ICAM-1 in HUVECs compared with si-Control group.Conclusion:CCL2 could affect
ICAM-1 expression in HUVECs, which may provide a theoretical basis for clarifying the mechanisms of atherosclerosis. |
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