| 王世平 徐光玉 孙秀斌 崔子连 王新生.精子特异性钙通道CatSper1 对精子线粒体呼吸与能量代谢的影响[J].,2016,16(27):5373-5376 |
| 精子特异性钙通道CatSper1 对精子线粒体呼吸与能量代谢的影响 |
| The Effects of CatSper1 on Mitochondrial Respiration and EnergyMetabolismin Sperm |
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| DOI： |
| 中文关键词: 精子 CatSper1 线粒体 ATP |
| 英文关键词: Sperm CatSper1 Mitochondria ATP |
| 基金项目:国家自然科学青年基金项目(81202016) |
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| 中文摘要: |
| 目的：应用CatSper1 单克隆抗体抑制CatSper1 的功能，检测精子线粒体呼吸功能及能量合成能力，观察CatSper1 对精子线
粒体呼吸与能量代谢的影响。方法：健康志愿者20 例，检测精液均符合WHO 健康标准。手淫法获取精液，经过上游法处理后每份
精液分为A、B 两组，分别与Earles液以及50 ug/mL抗CatSper1 多克隆抗体共孵育。在1 h，2 h，4 h后分别检测两组精子精液参
数、细胞线粒体呼吸控制率RCR及精子细胞ATP 含量。结果：与A 组比较，B 组精子各时间点a＋b(%)尤其是a(%)均明显下降，
差异具有统计学意义（P<0.01）；1 h后B组精子a(%)即明显下降，2 h，4 h后精子下降缓慢，与1 h相比无明显差异，无统计学意义
(P>0.05)。与A 组比较，B 组精子各时间点态3 值、呼吸控制率RCR 以及精子细胞ATP含量均明显下降，差异具有统计学意义
（P<0.01）；B 组精子中，2 h、4 h节点精子态3值、呼吸控制率RCR 以及精子细胞ATP含量与1 h无明显差异，无统计学意义(P>0.
05)。结论：阻断精子特异性钙通道CatSper1，可降低精子细胞线粒体呼吸功能，减少精子生成ATP的能力，从而使精子活力降低。
为精索静脉曲张引起精子CatSper1 表达下降，从而导致不育的机制寻找可能的理论依据。 |
| 英文摘要: |
| Objective:To observe the effects of CatSper1 on sperm mitochondrial respiration and energy metabolism by examining
RCR and ATP after the inhibition of CatSper1 by CatSper1 monoclonal antibodies.Methods:The semen from 20 volunteers were
conforming to standard of WHO, and were available by masturbation. Each sample was individually processed using a swim-up procedure
and were divided into two groups(group A,B). The sample in group A were incubated with Earles, while the sample in group B were
respectively incubated with anti CatSper1 monoclonal antibodies at a concentration of 50 ug/mL. We measured the semen parameters,
Cells mitochondrial respiratory control rate (RCR) and sperm ATP content.Results:Compared with group A, the percentage of sperm
motility with grade a+b, especially grade a in sperms was significantly decreased in group B at all time point（P<0.01）. It becomes small
of the descent rate ofa (%) at the time of 2 h, 4 h, and there were no difference in a (%) among the time point in groupB (P>0.05). The
state III, RCR and ATP content in spermwere significantly decreased in group B than that in group A（P<0.01）. But there were no difference
in state III, RCR and ATP content in sperm among the time point of 1 h, 2 h and 4 h in group B.Conclusion:Inhibition of CatSper1
can reduce the sperm vitality via hindering cells mitochondrial respiratory function and decreasing the ability to generate ATP in sperm.
The paper might find a new theory for the decrease of CatSper1 in spermand infertility caused by varicocele. |
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