| 周波 孙朝阳 夏萌 卢运萍 陈刚 徐洪斌.miR-9 在卵巢癌细胞上皮间质转化中的初步研究[J].,2016,16(31):6001-6004 |
| miR-9 在卵巢癌细胞上皮间质转化中的初步研究 |
| miR-9 Regulation of Epithelial-to-Mesenchymal Transition in OvarianCancer Cells |
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| DOI： |
| 中文关键词: miR-9 卵巢癌 侵袭与转移 E-cadherin |
| 英文关键词: miR-9 Ovarian cancer Invasion and migration E-cadherin |
| 基金项目:国家自然科学基金项目（81272859）；深圳科技计划项目（Jcyj20140416122811911；Jcyj20140416122811949） |
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| 中文摘要: |
| 目的：研究miR-9 在卵巢癌细胞上皮间质转化（EMT）中的作用。方法：上调或者下调miR-9 后，在RNA 水平上通过
RT-qPCR检测卵巢癌细胞系SKOV3 和A2780 中上皮指标E-cadherin 表达变化；在蛋白水平，通过western blotting 方法检测2 株
细胞系中上皮指标E-cadherin 和间质指标vimentin蛋白表达变化。生物信息学预测可能靶向E-cadherin 3'UTR 的miRNA，双荧
光素酶报告系统进一步验证miR-9 靶向结合E-cadherin 的3'UTR区。结果：上调miR-9 后，卵巢癌细胞系中E-cadherin 表达受到
明显抑制，vimentin 表达明显增加；反之，下调miR-9 后，E-cadherin 表达明显增高，vimentin 表达明显降低。通过生物信息学预测
发现miR-9可以直接靶向E-cadherin 的3'UTR 区，荧光素酶报告系统验证预测结果正确。结论：miR-9促进卵巢癌细胞上皮间质
转化。 |
| 英文摘要: |
| Objective:To study role of miR-9 in epithelial-to-mesenchymal transition (EMT) of ovarian cancer cells.Methods:RT-qPCR was used to detect the expression of E-cadherin after miR-9 mimics or inhibitor transfected in SKOV3 and A2780 cells at
RNA level. Western blotting was performed to detect the expression of E-cadherin and vimentin at protein level. Bioinformatics analysis
was conducted to predict the microRNAs that may regulate the 3' UTR of E-cadherin mRNA, which was further confirmed through Luciferase
reporter assay.Results:E-cadherin was significantly decreased after miR-9 mimics transfected in SKOV3 and A2780 cells both
at RNA and protein levels, while vimentin was markedly increased. However, these phenomena could be reversed after miR-9 inhibitor
administration. The 3' UTR of E-cadherin mRNA was predicted to be directly regulated by miR-9 via bioinformatics analysis. Luciferase
reporter assay was performed to further confirm that miR-9 could directly target the 3' UTR of E-cadherin mRNA.Conclusion:miR-9
may promote EMT in ovarian cancer cells. |
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