| 王 旭,李 娇,王 峰,谭 琰,闫 琰,张 琦,于 雪,范盎然,华 茜.三七、栀子有效组分给药的APP/PS1小鼠血清神经递质与行为学的相关性[J].,2017,17(1):11-15 |
| 三七、栀子有效组分给药的APP/PS1小鼠血清神经递质与行为学的相关性 |
| Effect of the Effective Components of Radix Notoginseng and Fructus Gardeniae and Their Combination on Serum ACh and 5-HT Levels of APP/PS1 Mice and Its Correlation with Mice Behavior |
| 投稿时间：2016-06-10 修订日期：2016-07-05 |
| DOI：10.13241/j.cnki.pmb.2017.01.003 |
| 中文关键词: 乙酰胆碱 三七总皂苷 栀子苷 阿尔茨海默病 行为学 |
| 英文关键词: Acetylcholine Panax notoginseng saponins Geniposide Alzheimer's disease Behavior |
| 基金项目:国家自然科学基金项目（81473546） |
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| 中文摘要: |
| 摘要 目的：探讨三七、栀子有效组分及配伍给药对APP/PS1小鼠血清中乙酰胆碱和五羟色胺含量的影响及其与行为学的相关性分析。方法：APP/PS1小鼠随机分成转基因模型组(Tg)、三七总皂苷组(PNS)、栀子苷组(GP)、配伍组(PNS+GP)，同窝非转基因小鼠为野生型组(WT)，从4月龄开始，以自主进食的方式，分组给药3个月，7月龄时，进行八臂迷宫测试和血清中乙酰胆碱及五羟色胺含量的检测。结果：①乙酰胆碱含量测定结果，与野生型组相比，转基因模型组有显著性降低（P<0.05）；三七总皂苷组、栀子苷组可以显著升高转基因模型组的乙酰胆碱含量（P<0.05）。②五羟色胺含量测定结果，与野生型组相比，转基因模型组有降低趋势，但无统计学意义（P>0.05）；三七总皂苷组、栀子苷组和配伍组对转基因组的五羟色胺含量有升高趋势，但都没有统计学意义（P>0.05）。③相关性分析结果，八臂迷宫测试中小鼠进入食物臂的时间百分比与血清中乙酰胆碱的含量成正相关（r=0.35，P＜0.05），与血清中五羟色胺的含量没有相关性（rs=0.16，P=0.39）。结论：三七、栀子有效组分及配伍给药对APP/PS1小鼠血清中乙酰胆碱的含量有调节作用，APP/PS1小鼠血清中乙酰胆碱的含量与八臂迷宫行为学测试体现的空间学习记忆能力成正相关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of the effective components of Radix Notoginseng and Fructus Gardeniae and their com- bination on the serum acetylcholine (ACh) and 5-Hydroxytryptamine (5-HT) levels of APP/PS1 mice and to analyze its correlation with the behavior of mice. Methods: APP/PS1 mice were randomly divided into transgenic model group (Tg), Panax notoginseng saponins group (PNS), geniposide group (GP), and compatibility group (PNS+GP), with non-transgenic littermate mice as wild-type group (WT). Four-month old mice were administered with corresponding drugs by self-feeding for 3 months, and at 7 months of age, 8-arm maze test were performed and the serum ACh and 5-HT contents were detected. Results: ①The results of ACh contents showed that the ACh con- tent of the Tg group was significantly decreased compared with the WT group (P<0.05); the ACh contents of the PNS and GP groups were significantly higher than that of the Tg group (P<0.05). ②The results of 5-HT contents showed that the content of 5-HT of the Tg group showed a decreasing trend compared with the WT group（P>0.05）; the 5-HT contents of the PNS, GP, and PNS+GP groups had in- creasing trends when compared with the Tg group（P>0.05）. ③ The correlation analysis results indicated that the percentage of time for mice entering the food arm of the 8-arm maze was positively correlated with the contents of serum ACh (r=0.353, P<0.05), with no corre- lation with the contents of serum 5-HT（rs=0.16, P=0.39）. Conclusion: The effective components of Radix Notoginseng and Fructus Gardeniae and their combination can regulate the serum ACh level of APP/PS1 mice, and the serum level of ACh are positively correlat- ed with the spatial learning and memory performance of the APP/PS1 mice revealed by the 8-arm maze behavioral test. |
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