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目的：研究模拟微重力对前成骨细胞的增殖作用及其分子机制。方法：利用2D 回转器模拟失重条件培养前成骨细胞 MC3T3-E1 24 小时；将p-AKT 激活剂SC79 加入细胞培养基后模拟失重条件下培养前成骨细胞MC3T3-E1 24 小时；利用 Western blot技术分别检测细胞增殖相关蛋白PCNA以及AKT、p-AKT 的表达变化情况。结果：①与对照组相比，模拟失重组前成骨细胞增殖受到抑制（P<0.01），p-AKT/AKT 比值减小（P<0.01）；②加入SC79 组与对照组相比，p-AKT/AKT 比值显著增加；③加入SC79 的模拟失重组（MG+SC79）与模拟失重组相比（MG），p-AKT/AKT 比值增加，PCNA 蛋白表达增加（P<0.01），成骨细胞增殖有所恢复。结论：模拟微重力可能通过抑制AKT 的磷酸化形式抑制前成骨细胞的增殖，加入p-AKT 激活剂可部分恢复前成骨细胞的增殖。

英文摘要:

Objective:To investigate the effect of simulated microgravity on the proliferation and the molecular mechanism of the osteoblast.Methods:2D rotator was used to simulate weightlessness condition. The MC3T3-E1 cells were divided into 4 groups: control group (CON), the simulated weightlessness group (MG), the control with SC79 group (CON+SC79) and simulated weightlessness treatment with SC79 group (MG+SC79). AKT phosphorylation activator SC79 was added into the cell culture medium before exposing to simulated weightlessness, then all the MC3T3-E1 cells were cultured for 24 h. Changes among MC3T3-E1 cells were detected by Western blot analysis, which including cell proliferation related protein PCNA, p-AKT and AKT.Results:① Compared with the CON group, cell proliferation of MG group is inhibited (P<0.01), and the ratio of p-AKT/AKT was decreased (P<0.01).② Compared with the CON group, the ratio of p-AKT/AKT of CON+SC79 group was raised. ③Compared with MG group, p-AKT / AKT ratio was increased, PCNA protein expression was increased partially (P <0.01), and osteoblast proliferation had been restored in the MG+SC79 group.Conclusion:Simulated microgravity can inhibit the proliferation of osteoblasts by inhibiting the phosphorylation of AKT. The proliferation of osteoblasts are partially restored by the addition of p-AKT activator.

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