祁 烁,李天天,何 昊,董 青,田劭丹,徐 玥,孙艳平,郑 蕾,陈志雄,陈信义.强的松对ITP模型小鼠血管活性物质调节作用研究[J].,2017,17(34):6601-6606 |
强的松对ITP模型小鼠血管活性物质调节作用研究 |
Study on the Regulation of Prednisone on Serum Vasoactive Substances in ITP Mice Models |
投稿时间:2017-06-21 修订日期:2017-07-14 |
DOI:10.13241/j.cnki.pmb.2017.34.001 |
中文关键词: 免疫性血小板减少症 β-内啡肽 血管活性肠肽 5-羟色胺 去甲肾上腺素 |
英文关键词: Immune thrombocytopenic purpura(ITP) β-endorphin(β-EP) Vasoactive intestinal peptide(VIP) 5-hydroxytryptamine(5-HT) Norepinephrine(NE) |
基金项目:国家重点基础研究发展计划(973计划)(2013CB531705) |
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中文摘要: |
摘要 目的:通过检测血清β-内啡肽(β-endorphin, β-EP)、血管活性肠肽(Vasoactive intestinal peptide, VIP)、5-羟色胺(5-hydroxytryptamine, 5-HT)、去甲肾上腺素(Norepinephrine, NE)的水平变化,探讨强的松干预免疫性血小板减少症(Idiopathic thrombocytopenic purpura, ITP)模型小鼠的潜在作用机制。方法:将60只BALB/c小鼠随机分为空白对照组、模型组与强的松组,每组20只。模型组、强的松组注射抗小鼠血小板血清(APS)复制ITP动物模型,强的松组第8天开始进行强的松干预;注射APS前、注射48小时、注射第8天、注射第12天、注射第15天动态检测血小板、白细胞、血红蛋白含量;实验结束后用酶联免疫吸附法(ELISA)检测β-EP、VIP、5-HT、NE含量。结果:①注射APS前,三组血小板、白细胞、血红蛋白含量无统计学差异(p>0.05)。②注射48小时,模型组、强的松组血小板计数、血红蛋白含量下降,与空白对照组比较,有统计学差异(p<0.05、p<0.01)。③注射第8天,模型组、强的松组血小板、白细胞计数处于最低水平,与空白对照组比较有统计学差异(p<0.05、p<0.01)。④注射第12天(即给予强的松第4天),模型组、强的松组血小板计数、血红蛋白值仍处于低水平,与空白对照组比较,有统计学差异(p<0.05、p<0.01)。⑤注射第15天,模型组血小板计数、血红蛋白值与空白对照组比较,有统计学差异(p<0.05、p<0.01);强的松组血小板计数、血红蛋白含量明显上升,与模型组比较,有统计学差异(p<0.05)。⑥实验结束后,与空白对照组比较,模型组血清VIP、5-HT检测值下调(p<0.05),β-EP 、NE检测值上调(p<0.05);与模型组比较,强的松组VIP、5-HT检测值上调(p<0.05、p<0.01),β-EP、NE无明显变化(p>0.05)。结论:对血清VIP、5-HT等血管活性物质的调控作用可能是强的松治疗ITP的潜在作用机制之一。 |
英文摘要: |
ABSTRACT Objective: Base on the variations of β-endorphin(β-EP), Vasoactive intestinal peptide(VIP), Serotonin(5-HT) and Norepinephrine(NE), investigate the potential mechanism of prednisone treating Immunology Thrombocytopenic Purpura (ITP) . Methods: 60 BALB/c mice were randomly divided into blank control group, model group and prednisone intervention group. ITP mice model was duplicated by injecting with GP-APS except blank control group. After ITP disease model successful established, prednisone is used in prednisone intervention group. The contents of β-EP, VIP, 5-HT and NE of ITP mice were detected by enzyme linked immunosorbent assay (ELISA). Results: Compared with the values in blank control group, the detection values of VIP and 5-HT in model group declined while the detection values of β-EP and NE increased. Compared with prednisone intervention group, the detection values of VIP and 5-HT in model group increased while the detection values of β-EP and NE showed no significant change. Conclusion: Regulating of serum vasoactive substances such as VIP and 5-HT could be one of the potential mechanism of prednisone treating Immunology Thrombocytopenic Purpura (ITP). |
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