| 黄敬文,王 景,安丽凤,段剑飞,代 宇,高宏伟.地龙蛋白对人增生性瘢痕成纤维细胞的作用研究[J].,2017,17(34):6626-6631 |
| 地龙蛋白对人增生性瘢痕成纤维细胞的作用研究 |
| Study on the Effect of Dragon Protein on Fibroblasts of Human Hypertrophic Paralysis |
| 投稿时间：2017-07-05 修订日期：2017-07-31 |
| DOI：10.13241/j.cnki.pmb.2017.34.005 |
| 中文关键词: 地龙 HSF细胞 分离纯化 MMP-1、9 |
| 英文关键词: Dragon HSF cells Isolation and purification MMP-1, 9 |
| 基金项目:黑龙江省自然科学基金面上项目（H2015023）；黑龙江省博士后管理站课题（LBH-Z13198）；黑龙江中医药大学博士创新基金项目（2013bs03） |
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| 中文摘要: |
| 摘要 目的：研究鲜地龙提取物对人增生性瘢痕成纤维细胞(HSF)增殖作用，及从其分离纯化的活性蛋白对基质金属蛋白酶（MMP-1、9）表达影响。方法：地龙提取物依次通过DEAE离子柱层析、葡聚糖凝胶柱层析分离纯化，应用HSF细胞模型，以活性为导向得到活性组份。获得样品组份进行部分理化性质实验，及对HSF细胞基质金属蛋白酶（MMP-1、9）表达影响。结果：地龙样品组份明显抑制HSF细胞增殖；样品经化学反应定性为蛋白，SDS-PAGE电泳法测定分子量为38kDa，pH值的平均值为7.82。分离纯化的DB-2样品使基质金属蛋白酶表达量下调，P<0.05。结论：地龙样品组份能够显著抑制HSF细胞增殖，具有开发成为治疗瘢痕药物的潜力。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of the extractive of fresh earthworm on the proliferation of human hypertrophic scar fibroblast(hHSF) and the effect of the isolated protein on the expression of matrix metalloproteinases (MMP-1, 9). Methods: The extractive of fresh earthworm was purified by DEAE ion column chromatography and dextran gel column chromatography. The active components were obtained by using HSF cell model. The obtained samples were used for the Physical properties and chemical properties experiment and the effects on the expression of matrix metalloproteinases (MMP-1, 9) in HSF cells. Results: The samples of fresh earthworm were significantly inhibited the proliferation of human hypertrophic scar fibroblast(hHSF). The sample was identified as a protein by chemical reaction, the molecular weight of SDS - PAGE electrophoresis was 38kDa, PH average value is 7.82. The purified DB-2 samples reduced the expression of matrix metalloproteinases,P<0.05. Conclusion: Fresh earthworm sample components can significantly inhibit human hypertrophic scar fibroblast(hHSF) proliferation, with the development of the potential for the treatment of scar drug. |
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