| 黄庆文,何 江,阙丽琳,陈 伊,李 文.程序性死亡配体-1在鼻咽癌组织中的表达及临床意义[J].,2018,(3):528-531 |
| 程序性死亡配体-1在鼻咽癌组织中的表达及临床意义 |
| Expression of Programmed Cell Death Ligand -1 in Nasopharyngeal Carcinoma and Its Clinical Significance |
| 投稿时间：2017-07-31 修订日期：2017-08-23 |
| DOI：10.13241/j.cnki.pmb.2018.03.028 |
| 中文关键词: 程序性死亡配体-1 鼻咽癌 表达 临床意义 |
| 英文关键词: Programmed cell death ligand -1 Nasopharyngeal carcinoma Expression Clinical significance |
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| 中文摘要: |
| 摘要 目的：探讨程序性死亡配体-1（PD-L1）在鼻咽癌组织中的表达及临床意义。方法：选取2015年7月-2017年6月期间在我院接受治疗的鼻咽癌患者333例，收集其鼻咽癌组织作为观察组标本，另选取同期在我院接受治疗的慢性鼻咽炎患者102例的鼻咽炎组织作为对照组标本。采用免疫组化法和逆转录-聚合酶链反应（RT-PCR）检测两组患者鼻咽组织中PD-L1蛋白和PD-L1mRNA的表达，并分析观察组患者鼻咽癌组织中PD-L1蛋白和PD-L1mRNA的表达与临床病理参数的关系。结果：对照组患者鼻咽炎组织中PD-L1蛋白的阳性率为0.00%（0/102），观察组患者鼻咽癌组织中PD-L1蛋白的阳性率为69.37%（231/333），两组PD-L1蛋白的阳性率比较差异有统计学意义（P<0.05）。RT-PCR结果显示，对照组患者鼻咽炎组织中未见PD-L1 mRNA表达，观察组患者鼻咽癌组织中PD-L1 mRNA的相对表达水平为（0.82±0.27），差异有统计学意义（P<0.05）。观察组患者鼻咽癌组织中PD-L1蛋白和PD-L1 mRNA的表达与年龄、性别无关（P>0.05），而TNM分期为III-IV期、有淋巴结转移、有吸烟史患者鼻咽癌组织中PD-L1蛋白阳性率和PD-L1 mRNA的表达均高于TNM分期I-II期、无淋巴结转移、无吸烟史患者（P<0.05）。结论：在鼻咽癌组织中PD-L1蛋白和PD-L1mRNA呈现高表达，且其表达与TNM分期、淋巴结转移、吸烟史有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression and clinical significance of programmed cell death ligand -1 (PD-L1) in na- sopharyngeal carcinoma (NPC). Methods: A total of 333 patients with nasopharyngeal carcinoma,who were treated in the First People's Hospital of Yulin from July 2015 to June 2017, were collected; the nasopharyngeal carcinoma tissues were collected as observation group, 102 cases of chronic pharyngitis during the same period were taken as control group.Immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of PD-L1 and PD-L1mRNA in the nasopharynx tis- sues of two groups. The relationship between the expression of PD-L1 protein and PD-L1mRNA and the clinicopathological parameters in the patients with nasopharyngeal carcinoma were analyzed. Results: The positive rate of PD-L1 protein in the nasal pharyngitis tissue of the control group was 0.00% (0/102), the positive rate of PD-L1 protein in the nasopharyngeal carcinoma tissues of the observation group was 69.37% (231/333); the positive rates of PD-L1 protein in the two groups were statistically significant(P<0.05). The results of RT-PCR showed that there were no expressions of PD-L1 and mRNA in the control group, the relative expression of PD-L1 and mRNA in the nasopharyngeal carcinoma tissues of the observation group was (0.82+0.27), the difference was statistically significant (P<0.05). The expressions of PD-L1 protein and PD-L1 mRNA in the nasopharyngeal carcinoma tissues of the observation group were not related to age and sex(P>0.05). The positive rate of PD-L1 protein and the expression of PD-L1 and mRNA in nasopharyngeal carcinoma tissues in patients with TNM stage III-IV, lymph node metastasis and history of smoking were higher than those in patients with TNM stage I-II, no lymph node metastasis, and no smoking history (P<0.05). Conclusion: The expression of PD-L1 protein and PD-L1mRNA is highly expressed in nasopharyngeal carcinoma,and its expression is related to TNM staging,lymph node metastasis and smoking history. |
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