| 薛 庆,王 佳,倪建鑫,李纪君,秦卫军,袁建林.L-左旋肌肽诱导人肾癌Caki-2细胞凋亡的体外研究[J].,2018,(4):634-638 |
| L-左旋肌肽诱导人肾癌Caki-2细胞凋亡的体外研究 |
| A Study on L-carnosine-induced Apoptosis in Kidney Cancer Caki-2 Cells in vitro |
| 投稿时间：2017-04-27 修订日期：2017-05-20 |
| DOI：10.13241/j.cnki.pmb.2018.04.007 |
| 中文关键词: L-左旋肌肽 肾癌 凋亡 增殖 |
| 英文关键词: L-carnosine Kidney cancer Apoptosis Proliferation |
| 基金项目:陕西省协同创新计划项目（2015XT-53） |
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| 中文摘要: |
| 摘要 目的：研究L-左旋肌肽诱导人肾癌Caki-2细胞凋亡的作用及可能机制。方法：常规培养肾癌Caki-2细胞，以1×104/mL的密度接种，取对数生长期Caki-2细胞分为L-左旋肌肽5、20、50 mM处理组和空白对照组，分别采用L-左旋肌肽5、20、50 mM和生理盐水处理24、48h后，采用MTT法检测各组Caki-2细胞的增殖情况，采用流式细胞法检测各组细胞周期和细胞凋亡情况，采用Western blot方法检测各组Caki-2细胞caspase-3、Bcl-2及HIF-1α蛋白的表达情况。结果：与空白对照组相比，L-左旋肌肽5、20、50 mM处理组在24 h和48 h的细胞存活率均显著降低(P<0.05)，且其对Caki-2细胞的生长抑制作用呈现明显的时间-剂量依赖关系，其中50 mM L-左旋肌肽处理Caki-2细胞48 h后，细胞存活率最低。L-左旋肌肽5、20、50 mM处理组在作用48 h后，Caki-2细胞随着L-左旋肌肽作用浓度的增加，Bcl-2和HIF-1α的蛋白表达水平依次降低(P<0.05)，而caspase-3蛋白表达水平逐次提高(P<0.05)。结论：L-左旋肌肽在体外可抑制人肾癌Caki-2细胞的增殖，并诱导人肾癌Caki-2细胞的凋亡，其作用机制可能与抑制HIF-1α表达及激活caspase-3表达相关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the L-carnosine-induced apoptosis in human kidney cancer Caki-2 cells vitro and its mechanism. Methods: Human kidney cancer Caki-2 cells were cultured to 1×104 / mL cell density and divided into 5, 20, 50 mM L-carnosine group and control group which were treated with 5, 20 50 mM L-carnosine and saline respectively. At 24, 48 h after treatment, MTT assay was used to detect the proliferation of Caki-2 cells, flow cytometry assay was conducted to detect the apoptosis and cell cycle of Caki-2 cells, western blot was performed to detect the protein expression of Bcl-2, HIF-1α and caspase-3. Results: MTT assay showed that 5, 20, 50 mM L-carnosine could inhibit the growth of Caki-2 cells at 24 h and 48 h after treatment in a time and dose dependent manner as compared with the control group, of the cell growth inhibition ratio was the highest in the 50 mM-treated group. After Caki-2 cells were treated with 5, 20, 50 mM L-carnosine and saline for 48 h, the protein expressions of Bcl-2, HIF-1α were gradually reduced and caspase-3 was increased correspondingly. Conclusion: L-carnosine could inhibit the proliferation and promote apoptosis of Caki-2 cells in vitro. The mechanism of which may be related to the inhibition of HIF-1 alpha signaling pathways and activation of caspase-3 signaling pathways. |
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