| 丁 虹,庞燕芬,唐雯静,吕 雯,周 磊.卵巢癌组织中UHRF1蛋白的表达及其与卵巢癌细胞增殖和侵袭的关系[J].,2018,(9):1642-1645 |
| 卵巢癌组织中UHRF1蛋白的表达及其与卵巢癌细胞增殖和侵袭的关系 |
| Expression of UHRF1 Protein in Ovarian Cancer Tissue and Its Correlation with the Proliferation and Invasion of Ovarian Cancer Cells |
| 投稿时间：2017-10-09 修订日期：2017-10-31 |
| DOI：10.13241/j.cnki.pmb.2018.09.009 |
| 中文关键词: 卵巢癌 泛素样含PHD和环指域1 增殖 侵袭 |
| 英文关键词: Ovarian cancer UHRF1 Proliferation Invasion |
| 基金项目:浙江省医药卫生科学研究基金项目(2009B081）；浙江省自然科学基金项目(Y2090319） |
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| 中文摘要: |
| 摘要 目的：研究卵巢癌组织中泛素样含PHD和环指域1(Ubiquitin-like with PHD and ring finger domains 1,UHRF1)蛋白的表达及UHRF1对卵巢癌细胞增殖、侵袭的影响。方法：选取卵巢癌组织和癌旁正常组织，采用蛋白印迹法(Western blot)检测其UHRF1的蛋白表达。体外培养卵巢癌SKOV-3细胞株，分别转染UHRF1的siRNA和阴性对照siRNA，采用CCK-8检测细胞活力，Tran- swell检测细胞侵袭能力，荧光定量聚合酶链式反应法(FQ-PCR)检测CyclinD1、CDK6、MMP2和MMP9的mRNA表达。结果：卵巢癌组织中UHRF1蛋白表达水平显著高于癌旁正常组织(P＜0.05)；与转染阴性对照siRNA的SKOV-3细胞相比，转染UHRF1的siRNA的SKOV-3细胞活力明显降低、侵袭细胞数目明显减少(P＜0.05)，且细胞中CyclinD1、CDK6、MMP2和MMP9基因的mRNA表达水平显著降低(P＜0.05)。结论：UHRF1蛋白在卵巢癌组织中呈高表达状态，且可促进卵巢癌细胞的增殖和侵袭。 |
| 英文摘要: |
| ABSTRACT Objective: To study the expression of ubiquitin-like with PHD and ring finger domains 1(UHRF1) protein in ovarian cancer tissue and the effect of UHRF1 on the proliferation and invasion of ovarian cancer cells. Methods: Ovarian cancer tissue and adja- cent normal tissue were collected, the expresion of UHRF1 protein were detected by Westerrn blot. Ovarian cancer SKOV-3 cell was cul- tured in vitro, UHRF1 siRNA and negative control siRNA were transfected. Then the cell viability was assayed by CCK-8 kit, cell inva- sion capability was assayed by Transwell. Expressions of CyclinD1, CDK6, MMP2 and MMP9 mRNA were assayed by FQ-PCR. Results: The expression of UHRF1 protein in ovarian cancer tissue was higher than that in adjacent normal tissue. The cell viability of SKOV-3 cells and number of migrated cells transfected by UHRF1siRNA was significantly lower than that of SKOV-3 cell transfected by negative control siRNA, the expressions of CyclinD1, CDK6, MMP2 and MMP9 mRNA were significantly decreased. Conclusion: The expression of UHRF1 protein was significantly increased in ovarian cancer; UHRF1 could promote the proliferation and invasion of ovarian cancer cell. |
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