| 王克甲,李小强,韩 夫,计 鹏,胡大海.rh-GH在促进大鼠创面愈合中的作用研究[J].,2018,(10):1882-1886 |
| rh-GH在促进大鼠创面愈合中的作用研究 |
| Research on the Role of rh-GH in Promoting Wound Healing in Rats |
| 投稿时间：2018-01-31 修订日期：2018-03-23 |
| DOI：10.13241/j.cnki.pmb.2018.10.015 |
| 中文关键词: 创面愈合 生长激素 表皮生长因子 成纤维细胞生长因子 血管内皮细胞生长因子 |
| 英文关键词: Wound healing Growth facter EGF FGF VEGF |
| 基金项目:国家自然科学基金项目（81501684） |
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| 中文摘要: |
| 摘要 目的：通过在大鼠背部创面周围局部注射rh-GH（重组人生长激素，Recombinant Human Growth Hormone）来调控创面局部GH（生长激素，Growth Factor）水平，观察GH在创面愈合中的作用，并对其可能机制进行进一步研究。方法：在SD大鼠背部制作创面，创周定期注射不同浓度rh-GH，观察并分析创面愈合速度差异；选取rh-GH的最适浓度进行创周注射并定期取材，以West- ern-Blot检测创周组织中GH蛋白水平，并以RT-PCR检测创周组织中EGF（表皮生长因子，Epidermal Growth Factor）、FGF（成纤维细胞生长因子，Fibroblast Growth Factor）、VEGF（血管内皮细胞生长因子，Vascular Endothelial Growth Factor）的表达变化。结果：三个注射不同剂量rh-Gh的实验组大鼠创面平均愈合时间由快至慢依次为16.5±1.5天、17.1±2.9天、18.5±1.5天，与注射生理盐水的对照组平均愈合时间21.7±2.3天相比均明显增快，差异有统计学意义（P<0.05）。创缘组织中GH的Western-Blot结果显示对照组GH水平在创面形成初期升高，但伤后4天开始GH表达水平即逐渐下降，而rh-GH注射组的GH水平在创面形成后逐渐升高，并维持GH在高表达水平。检测创周组织中EGF、FGF、VEGF的RNA转录水平，PCR结果显示实验组各生长因子的基因转录水平在伤后2天起即明显高于对照组，差异有统计学意义（P<0.05）。结论：1.创伤初期创面周围组织中GH表达水平呈现一过性升高然后逐渐下降的过程；2.创面周围注射rh-GH可以提高组织中GH水平，并减少创面愈合时间；3.GH可以提高皮肤组织中EGF、FGF、VEGF水平，间接促进创面上皮化，加快创面愈合。 |
| 英文摘要: |
| ABSTRACT Objective: To control the level of GH in the wound area by injecting the rh-GH around the wound on the back of rats and to investigate the effects and the mechanisms of GH on wound healing. Methods: The wounds were made on the back of SD rats. Different concentrations of rh-GH were injected around the wounds periodically. The differences of wound healing rate were observed and analyzed to determine the optimum concentration of rh-GH. The tissue around the wound was collected for detection. The level of GH protein was analyzed by Western-Blot, and the EGF, FGF and VEGF expression were detected by RT-PCR. Results: The averge healing time of wounds in three experimental groups with different doses of rh-Gh was 16.5±1.5 days, 17.1±2.9 days and 18.5±1.5 days. Compared with the control group injected with normal saline, the healing rate of rh-GH group was significantly faster, the differ- ence was statistically significant(P<0.05). The Western-Blot of GH in wound edge tissue showed that the GH level of control group in- creased at the initial stage of wound, but began to decrease at 4 days after injury. The GH level of rh-GH group increased and maintained at a high stage gradually after injury. The RT-PCR results showed that the transcription level of EGF, FGF, VEGF in rh-GH group were significantly higher than that in the control group from 2 days after injury, the difference was statistically significant (P<0.05). Conclusion: 1）. The expression of GH in the tissue around the wound in the early stage of trauma shows a transient increase and then a gradual de- crease; 2）. Injection of rh-GH around the wound can increase the level of GH in the tissue and reduce the wound healing time; 3）. GH can improve the level of EGF, FGF and VEGF in skin tissue, indirectly promote wound epithelization and accelerate wound healing. |
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