| 孙鲁旻,杨东红,王 凯,房玉镇,韩兆国,叶之慧.Micro-CT监测尼古丁对大鼠正畸牙移动过程中牙周改建的影响[J].,2018,(13):2448-2451 |
| Micro-CT监测尼古丁对大鼠正畸牙移动过程中牙周改建的影响 |
| Effect of Nicotine on the Peridentium during Orthodontic Tooth Movement in Rats Monitored by Micro-CT |
| 投稿时间：2018-03-05 修订日期：2018-03-28 |
| DOI：10.13241/j.cnki.pmb.2018.13.009 |
| 中文关键词: 尼古丁 正畸 Micro-CT 骨小梁 IL-17 |
| 英文关键词: Nicotine Orthodontic Micro-CT Trabecular bone IL-17 |
| 基金项目:黑龙江省教育厅基础研究项目（2016-KYYWF-0611） |
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| 中文摘要: |
| 摘要 目的：探讨不同摄取量的尼古丁对大鼠正畸过程牙周改建的影响。方法：选择120只雄性Wistar大鼠并将其随机分为四组：A组-空白对照，B组-正畸模型，C组-正畸并0.01 mg/mL尼古丁给药，D组-正畸并1 mg/mL尼古丁给药。分别于实验开始后第1、3、7、14、21天通过Micro-CT和HE染色观察模型牙齿移动距离和牙周组织改变并通过ELISA实验检测IL-17的表达。结果：Micro-CT扫描显示：正畸建模组相对于空白对照组在牙移动距离、骨体积分数、骨密度等指标均有明显变化，变化最大幅度发生在D组，B、C两组之间的差异没有统计学意义(P>0.05)。21天，D组移动距离达到0.80±0.06 mm，明显高于B、C组(P<0.05)。相较于空白对照组(A组)，B、C、D三组Micro-CT测量的骨体积分数、骨密度、骨小梁厚度均降低，D组骨密度值降至1108.36±8.86 mg/cm3。HE染色结果显示：D组在21天时破骨细胞增多并出现牙根吸收陷窝伴牙周膜纤维排列混乱；ELISA检测显示B、C组IL-17的含量在第7天时达到峰值，D组则在14天含量最高。结论：高浓度的尼古丁可加速正畸牙齿的移动速度及牙槽骨吸收，增加牙周组织中的破骨细胞及IL-17表达水平。 |
| 英文摘要: |
| ABSTRACT Objective: The study the effect of differentdosis of nicotine on the periodontium during orthodontic treatment in adult rats. Methods: 120 cases of Wistar male rats were segmented into four groups stochastically: A-control; B-orthodontic model group; C-orthodontic + 0.01 mg/kg nicotine group; D-orthodontic+1 mg/kg nicotine group. Micro-CT was used to measure tooth movement and the changes on microstructure of trabecular, the variety of periodontal microstructures and expression of IL-17 on day 1, 3, 7, 14 and 21 were detected and compared. Results: Micro-CT scanning showed that the orthodontic groups changed largely on observed indicators, such as tooth movement, BVF, and BMD, when compared to control groups. There were no significant changes between B and C groups (P>0.05), D group changed mostly. The tooth movement of D group reached 0.80 ± 0.06 mm, which was obviously higher than B and C groups at day 21 (P<0.05). The BVF, BMD and Tb.Th in all groups lessened apart from A group. HE staining of A - D groups indicated that the ligament of D group was disordering and more osteoclasts.The expression level of IL-17 reached its peak at day 7 in B, C groups, but it was at day 14 for D group. Conclusion: High-dose nicotine can not only accelerate the speed of tooth movement and the absorption of alveolar bone;in addition, but also increase the quantity of osteoclast and IL-17 expression. |
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