| 莫小辉,杨连娟,余 琦,章楚光,余 茜,吴 飞,李 瑾,郭 怡,谭 飞,赖永贤.流式细胞术检测外周血淋巴细胞诊断淋巴瘤的应用研究[J].,2018,(13):2488-2493 |
| 流式细胞术检测外周血淋巴细胞诊断淋巴瘤的应用研究 |
| The Application of Flow Cytometry (FCM) for Detecting Peripheral Blood Immunophenotype in Diagnosis of Lymphoma |
| 投稿时间：2018-01-12 修订日期：2018-02-09 |
| DOI：10.13241/j.cnki.pmb.2018.13.018 |
| 中文关键词: 皮肤淋巴瘤 流式细胞学技术 免疫组化 免疫表型 |
| 英文关键词: Cutaneous lymphoma Flow cytometry Immunohistochemistry Immunophenotype |
| 基金项目:国家自然科学基金项目（81502738）；上海市卫计委项目（20114Y057）；上海卫计委重点学科项目（12GWZX0902） |
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| 中文摘要: |
| 摘要 目的：探讨流式细胞术(FCM)检测外周血淋巴细胞在淋巴瘤诊断中的应用价值。方法：通过筛选2011年8月至2017年8月期间初诊的皮肤淋巴瘤病例25例，淋巴节良性病变6例，采用FCM检测外周血淋巴细胞表面抗原分子，通过与病理切片HE染色和免疫组化法（金标准）比较，分析两种检测方法之间的差异。结果：在31例检测病例中，FCM检测结果与金标准检测结果一致性较高(Kappa=0.61)：26例检查结果相同，5例检查结果不一致；检测19例T淋巴细胞淋巴瘤，FCM检测结果与金标准检测结果一致性也较高(Kappa=0.57)：检测14例初诊为T细胞淋巴瘤病例，FCM检测T淋巴瘤细胞的表面抗原标志CD3 分子为阳性，与组织学结果相符,另有5例T细胞淋巴瘤病例HE染色和免疫组化诊断明确，而FCM未能检出。检测6例B细胞淋巴瘤病例，6例淋巴瘤病例FCM检测结果都为阳性，FCM检测B淋巴瘤细胞的表面抗原标志CD19分子为阳性，与金标准检测结果符合率为100%。6例淋巴节良性病变病例FCM检测结果与金标准检测结果一致。结论：通过FCM检测外周血可以检测出部分皮肤淋巴瘤，FCM在皮肤淋巴瘤诊断和分型中有一定的临床价值，是检测皮肤淋巴瘤的有效的辅助方法。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze clinical value of flow cytometry (FCM) for detecting peripheral blood immunophenotype in diagnosis of cutaneous lymphoma. Methods: Peripheral blood and biopsy specimens from 25 persons who were suspected as cutaneous lymphoma and 6 persons with benign lesions of lymph node were collected. The comparison between FCM and HE staining & immunohistochemistry (IHC, gold standard) in diagnosis of lymphoma was made. Results: In all 31 cases, the FCM test results are in good agreement with the gold standard test results (Kappa=0.61): the results of the examination were the same in 26 cases, and the results of 5 cases were inconsistent. In the 19 TNHL(T cell non-Hodgkin lymphoma, TNHL) cases, the FCM test results are also in good agreement with the gold standard test results(Kappa=0.57). In the 19 cases of T cell lymphoma, the 14 cases of newly diagnosed T cell lymphoma were detected by FCM. Abnormal peripheral blood lymphocyte subsets could be detected by FCM. The surface antigen marker of T lymphoma cells from peripheral blood was positive for CD3 by FCM, which was consistent with histological findings. FCM and HE staining & IHC were equally effective for diagnosis of lymphoma in 14 patients. But FCM did not detected abnormal cells in 5 patient with T lymphoma. Abnormal peripheral blood lymphocyte subsets could be detected by FCM in the 6 cases of B cell lymphoma. The surface antigen marker of B lymphoma cells from peripheral blood was positive for CD19 by FCM, which was consistent with histological findings. In 6 cases with benign lesions of lymph node, the FCM test results are also in good agreement with the gold standard test results. Conclusion: The immunophenotye analysis by FCM was used to diagnose cutaneous lymphoma and provided an approach to increase sensitivity for diagnosis of lymphoma. |
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