| 荣 媛,刘明华,邓朝霞,任小宝,向 强.RNA干扰敲低β-catenin的表达对瘢痕疙瘩成纤维细胞增殖及凋亡的影响[J].,2018,(14):2631-2636 |
| RNA干扰敲低β-catenin的表达对瘢痕疙瘩成纤维细胞增殖及凋亡的影响 |
| Effects of β-catenin on the Proliferation and Apoptosis of Keloid Fibroblast Cells |
| 投稿时间：2017-12-28 修订日期：2018-01-23 |
| DOI：10.13241/j.cnki.pmb.2018.14.006 |
| 中文关键词: 瘢痕疙瘩 β-catenin 增殖 凋亡 |
| 英文关键词: Keloid β-catenin Proliferation Apoptosis |
| 基金项目:国家自然科学基金项目（81071537） |
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| 中文摘要: |
| 摘要 目的：探讨β-catenin对人瘢痕疙瘩成纤维细胞(keloid fibroblasts, KFB)增殖和凋亡的影响。方法：分别采用Real-time PCR(RT-PCR)和Western blot检测正常组织和瘢痕疙瘩中β-catenin的mRNA及蛋白表达。将针对人β-catenin 基因设计合成的3对特异性小干扰RNA(siRNA)分别转染体外培养的人瘢痕疙瘩成纤维细胞，通过RT-PCR和Western blot筛选出干扰人瘢痕成纤维细胞β-catenin 基因表达的最佳siRNA。通过siRNA沉默β-catenin表达后，采用MTT法检测KFB的增殖情况，流式细胞仪检测细胞凋亡率。结果：瘢痕疙瘩成纤维细胞中β-catenin的表达较正常组织明显升高(P＜0.05)。通过转染β-catenin siRNA降低其表达后，成纤维细胞的增殖能力明显下降(P＜0.05)，凋亡水平显著增高(P＜0.05)。结论：沉默β-catenin能够显著抑制瘢痕疙瘩成纤维细胞增殖并促进其凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of β-catenin siRNA on the proliferation and apoptosis of keloid fibroblast cell. Methods: Real-time PCR and Western blot were performed to monitor the mRNA and protein expression of β-catenin in pathological scar tissue and adjacent normal tissue. Keloid fibroblast cell in vitro were transfected with 3 pairs of specific β-catenin small interfering RNA (siRNA), then Real-time PCR and Western blot were performed to identify the best siRNA. The proliferation and apoptosis of KFB transfected with β-catenin siRNA were detected by using MTT assay and flow cytometry. Results: The RT-PCR and Western blot assay results showed that the expression of β-catenin mRNA and protein in pathological scar tissue were significantly higher than those in the adjacent normal tissue(P＜0.05). The β-catenin siRNA 2 remarkably inhibited the expression of β-catenin at mRNA and proteins in the Human keloid fibroblasts. Compared with the control group, cell proliferation was decreased and apoptotic rate were increased in β-catenin siRNA 2 group. Conclusion: Knockdown of β-catenin significantly decreased the proliferation and increased the apoptosis of KFB, which could inhibit the formation of pathological scar. |
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