| 吴 杰,郭 征,石 磊,李明辉,鲁亚杰,高 鹏,肖 鑫,杨 迪.纳米金壳介导的光热效应对骨肉瘤干细胞体外杀伤作用的研究[J].,2018,(20):3830-3834 |
| 纳米金壳介导的光热效应对骨肉瘤干细胞体外杀伤作用的研究 |
| In vitro Killing Effect of Gold Nanoshells-mediated Photothermal Therapy on Osteosarcoma Stem Cells |
| 投稿时间：2018-03-14 修订日期：2018-04-11 |
| DOI：10.13241/j.cnki.pmb.2018.20.006 |
| 中文关键词: 骨肉瘤 肿瘤干细胞 纳米金壳 光热疗法 |
| 英文关键词: Osteosarcoma Tumor stem cell Gold nanoshell Photothermal therapy |
| 基金项目:西京医院学科助推基础研究项目（XJZT15M06) |
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| 中文摘要: |
| 摘要 目的：探究纳米金壳介导的光热效应对骨肉瘤干细胞的杀伤作用。方法：采用无血清悬浮培养法富集骨肉瘤干细胞，通过实时荧光定量PCR 检测所富集细胞CD133、SOX2、NANOG、OCT4 mRNA的相对表达量以进行鉴定。将纳米金壳与骨肉瘤干细胞共培养，应用波长808 nm的近红外激光器 ( 1.5 W/cm2)进行照射以激发光热效应，分别用CCK8法检测光热疗法对骨肉瘤干细胞的增殖抑制率，Annexin C-Fitc/PI双染法检测光热疗法对骨肉瘤干细胞凋亡的影响。结果：成功富集了高表达CD133、SOX2、NANOG、OCT4 mRNA的骨肉瘤干细胞；纳米金壳介导的光热效应显著抑制了骨肉瘤干细胞的增殖率，当纳米金壳浓度在0~250 μg/mL范围内时，增殖抑制率与浓度呈正相关；细胞凋亡结果显示，纳米金壳结合近红外照射组细胞凋亡率显著高于对照组。结论：纳米金壳介导的光热效应对骨肉瘤干细胞增殖有明显抑制作用，主要诱导骨肉瘤干细胞发生中晚期凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the in vitro killing effect of gold nanoshells-mediated photothermal therapy. Methods: Sphere formation assays were used to isolate osteosarcoma stem cells, real - time fluorescence quota PCR were used to identify the stem cell by evaluate the expression of CD133, SOX2, NANOG, OCT4. osteosarcoma stem cells were co-cultured with different concentration of gold nanoshell, 1.5 W/cm2 near infrared with a wavelength of 808nm was used to irradiate the cell. CCK8 assay was used to detect the proliferation inhibition rates after treatment with photothermal therapy. Annexin-FITC／PI double staining was used to detect the apoptotic rates. Results: Osteosarcoma stem cells with high expression of CD133, SOX2, NANOG, OCT4 were isolated. Photothermal therapy significantly inhibited osteosarcoma stem cell proliferation. Concentration-dependent were observed when the concentration of gold nanoshell between 0~250 μg/mL. The apoptosis rate of photothermal therapy group was significantly high compare to control group. Conclusion: Photothermal therapy mediated by gold nanoshell can inhibit proliferation of osteosarcoma stem cell by mechanism of induction the apoptosis. |
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