| 肖明明,张瑞芳,李 灿,刘漪沦,刘卫华.不同能量CO2点阵激光对博来霉素诱导的小鼠增生性瘢痕的作用及Hedgehog信号通路的影响[J].,2019,19(2):244-248 |
| 不同能量CO2点阵激光对博来霉素诱导的小鼠增生性瘢痕的作用及Hedgehog信号通路的影响 |
| Effect of Fractional CO2 Laser on Mice Hypertrophic Scar and Hedgehog Pathway induced by Bleomycin |
| 投稿时间：2018-07-23 修订日期：2018-08-18 |
| DOI：10.13241/j.cnki.pmb.2019.02.009 |
| 中文关键词: 增生性瘢痕 CO2 点阵激光 组织病理 纤维化 GLi1 蛋白 |
| 英文关键词: Hypertrophic scar Fractional CO2 Laser Histopathology Fibrosis GLi1 protein |
| 基金项目:四川省科技计划项目(2017JY0304；2018054)；四川省医学会科研课题计划(S16021)；四川省卫计委重点项目（16ZD038） |
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| 中文摘要: |
| 摘要 目的：探讨不同能量CO2点阵激光对博莱霉素诱导的小鼠增生性瘢痕模型的作用及其对瘢痕组织中Hedgehog信号通路的影响。方法：于雄性C57BL/6J小鼠背部皮肤注射博来霉素（1 mg/d，4周）制作增生性瘢痕模型，另取4只小鼠背部注射PBS缓冲液作为对照。造模成功之后，随机将小鼠分为瘢痕对照组（模型组），10 mj激光治疗组（10 mj组）和20 mj激光治疗组（20 mj组），每组6只小鼠。10 mj组小鼠给予10 mj激光治疗（共3次，每次间隔2周）；20 mj组小鼠给予20 mj激光治疗（共3次，每次间隔2周）。治疗结束后，处死小鼠，取瘢痕全层标本进行病理组织学染色观察（HE、Masson染色）以及α-平滑肌肌动蛋白（α-SMA）、GLi1 免疫荧光观察。结果：①我们成功复制出小鼠增生性瘢痕模型；②20 mj CO2点阵激光治疗可有效修复瘢痕组织，经治疗后皮肤瘢痕程度显著减轻，同时可降低真皮层厚度和减轻瘢痕组织的纤维化程度；③免疫荧光染色结果提示，CO2点阵激光可显著减少小鼠皮肤增生性瘢痕中α-SMA、GLi1表达。结论：于小鼠的背部皮肤注射博莱霉素可建立增生性瘢痕模型。CO2点阵激光为治疗增生性瘢一种有效的治疗方式，其作用可能与其对Hedgehog信号通路的抑制有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of different energies fractional CO2 laser on bleomycin-induced mice hypertrophic scar model and Hedgehog signaling pathway in scar tissue. Methods: Bleomycin (1 mg/d, 4 weeks) was injected into the back skin of male C57BL/6J mice to establish hypertrophic scar model, and PBS was injected into the back of 4 mice as control. After the model was erected, the mice were randomly divided into scar control group (model group), 10 mj laser treatment group (10 mj group) and 20 mj laser treatment group (20 mj group), 6 mice in each group. Mice in 10 mj group were treated with 10 mj laser (three times, two weeks apart) and mice in 20 mj group were treated with 20 mj laser (three times, two weeks apart). At the end of treatment, the mice were sacri- ficed, and the full-thickness scar specimens tissues were taken for histopathological staining (HE, Masson's staining) and immunofluores- cence observation the expression of alpha-SMA and GLi1. Results: ① We successfully replicated the mouse model of hypertrophic scar. ② Fractional CO2 laser treatment can effectively repair scar tissue and reduce the thickness and fibrosis degree of dermis. ③ The results of immunofluorescence staining suggested that CO2 lattice laser could significantly reduce the expression of alpha-SMA and GLi1 in hy- pertrophic scars of mouse skin. Conclusion: A model of hypertrophic scar can be established by injecting bleomycin into the skin of the back of mice. CO2 lattice laser is an effective treatment for hypertrophic scar, and its effect may be related to the inhibition of Hedgehog signaling pathway. |
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