| 吴 敏,陈俊梅,刘召波,刘海东,刘 超,张爱英,李 宁.乙型肝炎病毒核心抗体IgM的蛋白芯片检测法研究[J].,2019,19(3):560-562 |
| 乙型肝炎病毒核心抗体IgM的蛋白芯片检测法研究 |
| Detection of Human Anti-hepatitis B Virus Core Antibody IgM by Protein Microarray |
| 投稿时间：2018-05-22 修订日期：2018-06-18 |
| DOI：10.13241/j.cnki.pmb.2019.03.038 |
| 中文关键词: 蛋白芯片 乙肝病毒 定性 抗HBc-IgM |
| 英文关键词: Protein Microarray Hepatitis B virus Qualitative Anti-HBc IgM |
| 基金项目:北京市科技委员会基金项目(Z181100001018031) |
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| 中文摘要: |
| 摘要 目的：探索蛋白芯片技术检测乙型肝炎病毒（HBV）抗HBc-IgM的可行性。方法：应用Nano-Plotter TM-压电式微量喷墨点阵制备系统自制的核心抗原蛋白芯片在经10%山羊血清封闭后加入待测血清37℃孵育，PBST清洗晾干后后再次加入检测抗体即HRP-抗人IgM抗体，芯片用伯乐成像仪检测有无信号。结果：24份HBsAg和抗HBc阳性血清中经蛋白芯片检测抗HBc-IgM阳性率为83.3%（20/24）；24份健康志愿者血清中经蛋白芯片检测抗HBc-IgM阳性率为4.1%（1/24）。乙肝血清与健康志愿者血清化学发光信号差异明显。结论：蛋白芯片技术可较好地应用于定性检测HBV抗HBc-IgM，为临床快速判断是否存在HBV感染和监测慢性肝炎HBV活动性提供新的辅助诊断方法。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the feasibility of protein microarray for detecting human anti-hepatitis B virus core antibody IgM qualitatively. Methods: Protein microarray was fabricated by GeSiM Nano-PlotterTM Micropipetting System. The core antigen protein spotted on the slides with BSA spotted as control protein. All slides were blocked in a coupling buffer (10% normal goat serum with 0.1%NaN3). Then serum samples were added on the slides and incubated for 2h at 37℃. After rinsed by PBST, the slides were added with HRP-anti-human IgM antibody and then scanned by a chemiluminescent scanner, Chemi DocTM MP System (Bio-Rad, California, USA). Results: The positive rate of anti-HBc IgM was 83.3% (20/24) in 24 HBsAg and anti-HBc positive serum samples detected by protein microarray, while the positive rate of anti-HBc IgM was 4.1% (1/24) in 24 healthy volunteers. The chemiluminescent signal difference was significant between hepatitis B positive and healthy serum samples. Conclusion: Protein microarray technology could be applied to detect anti-HBc IgM qualitatively, and provide a new auxiliary diagnostic method for clinical rapid determination of HBV infection and monitoring HBV chronic hepatitis activity. |
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