| 魏 潇,倪江东,宋德业,郭双飞,朱 卫.加味四妙散对尿酸钠诱导急性痛风性关节炎大鼠的疗效机制研究[J].,2019,19(7):1257-1260 |
| 加味四妙散对尿酸钠诱导急性痛风性关节炎大鼠的疗效机制研究 |
| Study on the Therapeutic Mechanism of Jiawei Simiao Powder on Rats with Acute Gouty Arthritis Induced by Sodium Urate |
| 投稿时间：2018-11-03 修订日期：2018-11-27 |
| DOI：10.13241/j.cnki.pmb.2019.07.011 |
| 中文关键词: 加味四妙散 急性痛风性关节炎 大鼠 疗效 |
| 英文关键词: Jiawei Simiao powder Acute gouty arthritis Rat Efficacy |
| 基金项目:湖南省科技创新项目（2018JJ1046） |
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| 中文摘要: |
| 摘要 目的：研究急性痛风性关节炎大鼠应用加味四妙散的疗效，并研究其作用机制。方法：将60只雄性Wistar大鼠按照随机数字法随机分为5组，每组各12只，分组情况为：空白对照组、模型对照组、低剂量组（8.875 g/kg·d）、中剂量组（17.750 g/kg·d）及高剂量组（35.500 g/kg·d）。模型建立后每天灌胃治疗，持续3 d。计算并比较各组大鼠造模后4 h、24 h、48 h、72 h时踝关节的肿胀度。检测并比较各组大鼠血清白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）及尿酸（UA）水平。结果：（1）造模24h后，模型对照组大鼠关节肿胀度达到峰值，并且明显大于空白对照组，具有显著性差异（P<0.05）；模型对照组大鼠关节肿胀度明显大于各剂量组，同时高剂量组大鼠关节肿胀度明显小于中剂量组和低剂量组，均具有显著性差异（P<0.05）。造模48 h后，各组大鼠关节肿胀度均继续下降，且各剂量组大鼠关节肿胀度明显小于模型对照组，均具有显著性差异（P<0.05）。造模72 h后，各治疗组大鼠关节肿胀度均明显小于模型对照组，同时高剂量组大鼠关节肿胀度明显小于中剂量组和低剂量组，均具有显著性差异（P<0.05）。（2）与空白对照组比较，模型对照组大鼠血清IL-1β、TNF-α及UA水平均明显偏高，具有显著性差异（P<0.05）；与模型对照组、低剂量组比较，中剂量组和高剂量组大鼠血清IL-1β、TNF-α及UA水平均明显偏低，具有显著性差异（P<0.05）。结论：加味四妙散35.500 g/kg剂量能明显改善急性痛风性关节炎大鼠症状，减轻踝关节肿胀度，降低血清IL-1β、TNF-α及UA水平，对急性痛风性关节炎大鼠具有治疗作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of Jiawei Simiao powder on acute gouty arthritis in rats, and study its mechanism. Methods: 60 male Wistar rats were randomly divided into five groups, 12 in each group. The grouping was as follows: blank control group, model control group, low dose group (8.875 g/kg·d), middle dose group (17.750 g/kg·d) and high dose group (35.500 g/kg·d). After model es- tablishment, the rats were lavaged for three days. The swelling degree of ankle joint was calculated and compared at 4 h, 24 h, 48 h and 72 h after model establishment in rats of each group. The serum levels of interleukine-1β(IL-1β), tumor necrosis factor-α (TNF-α) and uric acid (UA) were measured and compared in each group. Results: (1)After 24 hours, the swelling degree of joint in the model group was reach the peak, and which was significantly higher than that in the blank control group, the difference was significant (P<0.05). The swelling degree of joint in model control group was significantly higher than that in each dose group, and the swelling degree of joint in high dose group was significantly lower than that in middle dose group and low dose group, the difference was significant (P<0.05). After 48 hours, the swelling degree of joint in each group continued to decrease, and the swelling degree of joint in each dose group was signif- icantly lower than that in the model control group, the difference was significant (P<0.05). After 72 hours, the swelling degree of joint in each treatment group was significantly lower than that in the model control group, and the swelling degree of joint in the high dose group was significantly lower than that in the middle dose group and the low dose group, the difference was significant (P<0.05). (2) Compared with the blank control group, the serum levels of IL-1β, TNF-α and UA in the model control group were significantly higher, the differ- ence was significant(P<0.05). Compared with model control group and low dose group, the serum levels of IL-1β, TNF-α and UA in middle dose group and high dose group were significantly lower, the difference was significant (P<0.05). Conclusion: Jiawei Simiao powder 35.500 g/kg can significantly improve the symptoms of acute gouty arthritis rats, reduce ankle swelling, reduce serum levels of IL-1β, TNF-α and UA, and it has therapeutic effect on acute gouty arthritis rats. |
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