| 陈富波,祁胜财,张 旭,谢云峰,吴津津,汪饶饶.豆浆作为脱位牙保存液对牙周膜成纤维细胞活性的影响研究[J].,2019,19(8):1416-1419 |
| 豆浆作为脱位牙保存液对牙周膜成纤维细胞活性的影响研究 |
| Viability of Human Periodontal Ligament Fibroblasts in Soybean Milk as Storage Media |
| 投稿时间：2018-08-23 修订日期：2018-09-18 |
| DOI：10.13241/j.cnki.pmb.2019.08.004 |
| 中文关键词: 豆浆 牙周膜成纤维细胞 保存液 |
| 英文关键词: Soybean milk Human periodontal ligament fibroblasts Storage media |
| 基金项目:国家自然科学基金项目(81500806) |
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| 摘要点击次数: 738 |
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| 中文摘要: |
| 摘要 目的：通过探讨牙周膜成纤维细胞保存在豆浆中对其细胞活性的影响，评价豆浆作为脱位牙保存液的可行性。方法：将因口腔正畸而拔除12-18岁青少年的前磨牙进行细胞分离，选用分离出来的牙周膜成纤维细胞进行培养，将其分别置于ViaSpan、DMEM高糖培养基、豆浆、自来水、牛奶、Hank平衡盐溶液这6种液体中进行培养，采用cck-8法测定其在不同保存液中1h、2h、4h、8h、24h五个时间点的细胞活性。结果：豆浆组1h、2h、4h、8h、24h细胞残存率都显著高于自来水组(P<0.01)；豆浆组1h、2h、4h、8h细胞残存率与DMEM组、ViaSpan组比较均无统计学差异(P>0.05)，在24h时的细胞残存率显著高于DMEM组和ViaSpan组(P<0.05)；豆浆组2h、8h细胞残存率和牛奶组比较无统计学差异(P>0.05)，但牛奶组1h、4h、24h细胞残存率显著高于豆浆组(P<0.05)；豆浆组1h、2h、4h、8h、24h细胞残存率和HBSS组比较均无统计学差异(P>0.05)。结论：豆浆在保存牙周膜成纤维细胞活性效果等效于Hank平衡盐溶液，是一种有效的脱位牙保存液。 |
| 英文摘要: |
| ABSTRACT Objective: To evaluate the feasibility of soybean milk as a storage media for avulsed teeth through analyzing the effect of soybean milk on the cell viability of periodontal ligament fibroblasts preserved in soybean milk. Methods: Human periodontal ligament fibroblasts cells were isolated from the premolars of 12-18 year-old adolescents after orthodontic extraction and cultured in ViaSpan, DMEM high glucose medium, soybean milk, tap water, milk and Hank balanced salt solution. Then the cell activity was measured by CCK-8 method at 1h, 2h, 4h, 8h, 24h in different storage solutions. Results: The cell survival rates of soybean milk group at 1h, 2h, 4h, 8h and 24h were significantly higher than those of tap water group(P<0.01). Compared with DMEM and ViaSpan groups, the cell survival rates of soybean milk group at 1h, 2h, 4H and 8h had no significant difference (P>0.05), and the cell survival rate at 24h was significantly higher than that of DMEM and ViaSpan groups (P<0.05). There was no significant difference between the soybean milk group and the milk group at 2h, 8h (P>0.05), but the cell survival rates of the milk group were significantly higher than that of the soybean milk group at 1h, 4h, 24h (P<0.05). There was no significant difference in cell survival rate between the soybean milk group and HBSS group at 1h, 2h, 4h, 8h and 24h (P>0.05). Conclusion: Soybean milk has the same effect as HBSS in maintaining the viability of human periodontal ligament fibroblasts, which can be recommended as a suitable storage media. |
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