| 翟佳佳,王 辛,张 艺,李 茉,路延之,康 健,邹 健,李泽平,李晓苗.小檗碱对损伤胰岛βTC6细胞相关信号分子表达的影响[J].,2019,19(10):1835-1839 |
| 小檗碱对损伤胰岛βTC6细胞相关信号分子表达的影响 |
| Effects of Berberine on the Expression of Signaling Molecules related with the Protection of Injured Pancreatic βTC6 Cells |
| 投稿时间：2019-01-17 修订日期：2019-02-15 |
| DOI：10.13241/j.cnki.pmb.2019.10.007 |
| 中文关键词: 小檗碱 棕榈酸 βTC6细胞 AMPK PTEN |
| 英文关键词: Berberine Palmic acid Pancreatic βTC6 cells AMPK PTEN |
| 基金项目:国家自然科学基金项目（81573746） |
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| 中文摘要: |
| 摘要 目的：探讨小檗碱在棕榈酸（palmic acid, PA）诱导的胰岛β细胞发生凋亡时PTEN及AMPK的变化及其作用机制。方法：采用1.0 mmol/L PA诱导胰岛?茁TC6细胞损伤模型，然后给予小檗碱干预。采用流式细胞术检测βTC6细胞凋亡率，RT-qPCR法及免疫荧光法检测在PA及小檗碱作用下信号转导通路分子PTEN及AMPK表达的变化。结果：PA抑制βTC6细胞的生长并诱导其凋亡，小檗碱可缓解PA引起的βTC6的凋亡，对氧化应激具有保护作用；伴随着凋亡的发生，AMPK的表达显著降低，而PTEN的表达显著升高，表明细胞氧化损伤严重时抑制了AMPK的表达及转录，而PTEN在βTC6细胞的凋亡中具有促进作用；小檗碱治疗后， AMPK的表达增多，而PTEN的表达下降，暗示着小檗碱通过激活AMPK并抑制PTEN的表达来增强βTC6细胞的抗氧化能力。结论：小檗碱对PA引起的细胞凋亡有保护作用，同时小檗碱可能通过调节AMPK及PTEN的表达来发挥抗氧化作用。 |
| 英文摘要: |
| ABSTRACT Objective: The aim of this study was to explore the protective effect of berberine (BBR) on the apoptosis of pancreatic βTC6 cells induced by palmic acid (PA) as well as investigate the role of PTEN and AMPK in BBR involved βTC6 cells protection. Methods: The injury of βTC6 cells was induced by treatment with 1.0 mmol/l PA, and then treated with BBR. The apoptosis of βTC6 cells was evaluated using flow cytometry. The expressions of AMPK and PTEN were estimated utilizing RT-qPCR and immunofluorescence. Results: The proliferation of βTC6 cells was significantly inhibited in PA group compared with that in Con group. Meanwhile, βTC6 cells apoptosis could be induced by PA treatment while BBR attenuated this effect, indicating that BBR played a protective role in the oxidative stress of βTC6 cells. During the occurrence of βTC6 cells apoptosis, the AMPK expression was dramatically reduced while the PTEN expression was significantly increased, suggesting that the expression and transcription of AMPK were inhibited by the severe oxidative damage of βTC6 cells. Besides, PTEN promoted the apoptosis of βTC6 cells. Compared with the PA group, the AMPK expression was increased while the PTEN expression was declined in PA+BBR group, indicating BBR enhanced the anti-oxidative ability of βTC6 cells by activating the AMPK expression and inhibiting the PTEN expression. Conclusion: BBR played a protective effect on PA-induced βTC6 cells apoptosis, and it showed anti-oxidative ability through regulating the expressions of AMPK and PTEN. |
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