| 张 健,王 巨,廖 飞,孙天浩,王 磊,曲俊峰,隋嘉泽,赵智宏,刘宏宇.香鳞毛蕨苷提取物E对人肺癌A549细胞增殖及凋亡的影响[J].,2019,19(12):2262-2266 |
| 香鳞毛蕨苷提取物E对人肺癌A549细胞增殖及凋亡的影响 |
| Effect of Compound E from Dryopteris Fragrans (L.) Schott on the Proliferation and Apoptosis of Human Lung Cancer A549 Cells |
| 投稿时间：2019-01-08 修订日期：2019-01-31 |
| DOI：10.13241/j.cnki.pmb.2019.12.013 |
| 中文关键词: 香鳞毛蕨苷提取物E 肺癌 增殖 凋亡 |
| 英文关键词: Fragranoside E Lung cancer Proliferation Apoptosis |
| 基金项目:黑龙江省卫生健康委科研项目(2014-270)；哈尔滨医科大学第一临床医院资助科研项目(2014Y003) |
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| 中文摘要: |
| 摘要 目的：研究香鳞毛蕨苷提取物E(Fragranoside E)对人肺癌A549细胞增殖及凋亡的影响。方法：将体外培养的人肺癌A549细胞分为对照组(0.1 %DMSO、100 nM紫杉醇)和实验组，通过CCK-8实验、克隆形成实验检测细胞增殖情况；检测乳酸脱氢酶(LDH)水平以探讨Fragranoside E的细胞毒性；透射电镜及流式细胞术检测A549细胞的凋亡情况。进一步采用5mM N-乙酰半胱氨酸(N-acetylcysteine，NAC)预处理A549细胞2 h后，采用荧光显微镜观察细胞内ROS的释放情况。结果：CCK8及克隆形成实验结果提示Fragranoside E呈浓度和时间依赖性抑制A549细胞增殖(P<0.05)；≤40 μM Fragranoside E处理A549细胞对其LDH的释放无显著影响(P>0.05)；而40 μM Fragranoside E可诱导A549细胞凋亡，细胞内ROS升高，NAC(5 mM)预处理2 h后，细胞内ROS(112.6 %±12.3 %)较Fragranoside E处理组显著降低(P<0.05)。结论：Fragranoside E能够抑制A549细胞增殖，诱导其凋亡，其抗肿瘤活性可能与细胞内ROS释放有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the efftect of Fragranoside E from Dryopteris fragrans (L.) Schott on the proliferation and apoptosis of human lung cancer of A549 cells. Methods: Human lung cancer A549 cells cultured in vitro were divided into the control group (0.1 %DMSO, 100 nM paclitaxel) and the Fragranoside E group. Cell proliferation was examined by Cell counting kit (CCK-8) assay and clone formation assay; cytotoxicity was tested by LDH assay; cell apoptosis was detected by transmission electron microscope and flow cytometry assay; releasing of ROS was observed by fluorescence microscopy, after some cells were pretreated with N-acetylcysteine (NAC) for 2 h. Results: The results of CCK8 and clone formation experiments suggested that Fragranoside E inhibited the proliferation of A549 cells in a dose-dependent and time-dependent manner(P<0.05), without any obvious cytotoxic below 40 μM concentration in LDH assay. Fragranoside E(40 μM) induced A549 cell apoptosis and the increasing of ROS, which was significantly reduced (112.6 %±12.3 %) in cells pretreated with NAC (P<0.05). Conclusion: Fragranoside E inhibited the proliferation and induced the apoptosis of A549 cells, which might be closely related to the releasing of ROS. |
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