| 李俊义,李秀梅,万京明,刘 娟,毕京玉,姜 彦,李 娜.BMSCs移植对视神经损伤家猫RGCs损伤及BDNF表达的影响[J].,2019,19(18):3438-3442 |
| BMSCs移植对视神经损伤家猫RGCs损伤及BDNF表达的影响 |
| Influence of Bone Marrow Mesenchymal Stem Cells Transplantation on the Retinal Ganglion Cells and Brain Derived Neurotrophic Factor after Traumatic Optic Neuropathy in Cats |
| 投稿时间：2019-03-23 修订日期：2019-04-18 |
| DOI：10.13241/j.cnki.pmb.2019.18.008 |
| 中文关键词: 骨髓间充质干细胞 视神经损伤 移植 视网膜神经节细胞 脑源性神经营养因子 |
| 英文关键词: Bone marrow mesenchymal stem cells Optic nerve injury Transplantation Retinal ganglion cells Brain derived neurotrophic factor |
| 基金项目:国家自然科学基金项目(81170895) |
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| 中文摘要: |
| 摘要 目的：探讨玻璃体腔内注射移植体外培养的骨髓间充质干细胞(Bone marrow mesenchymal stem cells, BMSCs)对家猫视神经损伤后视网膜神经节细胞(Retinal ganglion cells, RGCs)的影响及其可能的作用机制。方法：参照标准化家猫外伤性视神经损伤动物模型建立的方法建立右眼视神经夹伤家猫模型，然后将其分为以下四组：(1)A组：右眼BMSCs注射移植组，玻璃体腔内接受注射移植BMSCs浓度为1×105细胞/μL的单细胞悬液0.1 mL；(2)B组：右眼PBS注射组，玻璃体腔内注射PBS 缓冲液0.1 mL；(3)C组：假损伤控制组，BMSCs左眼组，仅暴露视神经而不损伤，不接受治疗；(4)D组：正常对照组，PBS左眼组，正常眼，不做任何处理。分别在移植后的3、7、14及28天，用免疫荧光染色双十八烷基四甲基吲哚羰基花青高氯酸盐染色标记法观察分离视网膜的RGCs存活率，用双抗体一步夹心法酶联免疫吸附试验方法检测分离视网膜的脑源性神经营养因子(Brain derived neurotrophic factor, BDNF)的含量。结果： 术后3、7、14及28天，在周边区及中央区视网膜上RGCs密度均显著减少(周边区：P3d=0.0446, P7d = 0.0011, P14d ＜0.001, P28d＜0.001；中央区：P3d =0.0437, P7d =0.0067, P14d＜0.001, P28d＜0.001)。7天、14天、28天后，A组RGCs密度及BDNF含量均显著高于B组(P＜0.05)。结论：BMSCs移植可以减缓外伤性视神经损伤家猫RGCs凋亡，可能与其增加BDNF表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of intravitreal injection of bone marrow mesenchymal stem cells(BMSCs) cultured in vitro on the retinal ganglion cells after optic nerve injury in cats and its possible mechanisms. Methods: A cat model of right optic nerve injury was established and divided into four groups: (1)Group A: Right eye BMSCs injection transplantation group, intravitreal injection transplantation BMSCs concentration of 1×105 cells/mL single cell suspension 0.1 mL;(2)Group B: right eye PBS injection group, intravitreal PBS buffer 0.1 mL;(3)Group C: Sham-injured control group, BMSCs left eye group, only exposed optic nerve without injury, no treatment;(4)Group D: normal control group, PBS left eye group, normal eyes, without any treatment. The survival rate of RGCs isolated from retina was observed by immunofluorescence staining with dicarboxyl tetramethylindole carbonyl cyanide perchlorate staining at 3, 7, 14 and 28 days after transplantation. The content of brain-derived neurotrophic factor in isolated retina was detected by double antibody sandwich ELISA. Results: At 3, 7, 14 and 28 days after operation, the RGCs density in peripheral and central retina decreased significantly (peripheral area: P3d = 0.0446, P7d = 0.0011, P14d < 0.001, P28d < 0.001; central area: P3d = 0.0437, P7d = 0.0067, P14d < 0.001, P28d < 0.001). After 7 days, 14 days and 28 days, RGCs density and BDNF content in group A were significantly higher than those in group B (P < 0.05). Conclusion: BMSCs transplantation can slow down the apoptosis of RGCs in cats with traumatic optic nerve injury, which may be related to the increase of BDNF expression. |
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