| 林昌岫,金成华,马天泽,朴成日,刘熙文,黄成日,黄京华.枸杞子水提取物对Bel-7402人肝癌细胞增殖的影响[J].,2019,19(19):3639-3643 |
| 枸杞子水提取物对Bel-7402人肝癌细胞增殖的影响 |
| Effect of Lycium barbarum Water Extract on Proliferation of Bel-7402 Human Liver Cancer Cell |
| 投稿时间：2018-12-30 修订日期：2019-01-25 |
| DOI：10.13241/j.cnki.pmb.2019.19.009 |
| 中文关键词: 枸杞子水提取物 Bel-7402人肝癌细胞 增殖 影响 |
| 英文关键词: Water extract of Lycium barbarum Bel-7402 human liver cancer cell Proliferation Effect |
| 基金项目:国家自然科学基金项目（81660478） |
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| 中文摘要: |
| 摘要 目的：探讨枸杞水提取物对Bel-7402人肝癌细胞增殖的影响。方法：用RPMI-1640完全培养液温育Bel-7402人肝癌细胞，分为空白对照组和药物组，空白对照组给予培养液处理，药物组分为低剂量组（100 μg/mL）和高剂量组（200 μg/mL），实验过程加入相应剂量的枸杞子水提取物。于倒置显微镜下观察三组细胞形态学变化，采用流式细胞仪观察细胞的凋亡状况及活性氧簇（ROS）含量，采用MTT法检测细胞增殖的抑制作用，采用反转录-聚合酶链式反应（RT-PCR）检测Bel-7402细胞中bax mRNA和bcl-2 mRNA的表达。结果：在倒置显微镜下观察，枸杞子水提取物使Bel-7402细胞生长受到抑制，表现为细胞贴壁减少、脱落增加，脱落的细胞浮悬于培养液中，呈圆形，体积缩小。枸杞子水提取物可促进Bel-7402细胞凋亡，药物组Bel-7402细胞的凋亡率比空白对照组明显增加(P<0.01)，G0/G1期细胞比率减少(P<0.05)，G2/M2期细胞比率增加(P<0.05)，且ROS含量增加(P<0.05)。枸杞子水提取物对Bel-7402细胞的生长有明显抑制作用，且抑制率随着培养时间的延长而增加，且高剂量组在96小时时抑制率最高。药物组Bel-7402细胞中bax基因灰度比值显著高于空白对照组(P<0.01)，bcl-2基因灰度比值显著低于空白对照组(P<0.01)。结论：枸杞子水提取物可抑制Bel-7402人肝癌细胞的增殖、阻滞细胞周期、诱导细胞凋亡，增加ROS含量，促进bax基因表达，抑制bcl-2基因表达，该实验结果可为枸杞子抗肝癌作用提供实验依据。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of Lycium barbarum water extract on proliferation of Bel-7402 human liver cancer cell. Methods: Incubated the Bel-7402 human liver cancer cells with RPMI-1640 culture completely, and they were divided into blank control group and drug group. The blank control group was treated with culture medium. The drug group were divided into low dose group (100 μg/mL) and high dose group (200 μg/mL), and the Lycium barbarum water extract at corresponding dose was added in the experiment. Observed the cells' morphological changes under inverted microscope, tested the apoptosis of cell and ROS level by flow cytometry instrument, detected cell proliferation inhibition by MTT, the levels of bax mRNA and bcl-2 mRNA expressions were detected by reverse transcription polymerase chain reaction(RT-PCR). Results: Cells growth were inhibited by adding Lycium barbarum water extract through inverted microscope, showed the adherent cells were reduced, abscission was increased, which floated in culture, round and smaller. Lycium barbarum water extract could promote the Bel-7402 cells apoptosis. The apoptotic rate of Bel-7402 cells in the drug group was significantly higher than that in the blank control group (P<0.01), G0/G1 phase cells ratio was decreased(P<0.05), G2/M2 phase cell ratio was increased(P<0.05), and level of ROS was increased(P<0.05). Lycium barbarum water extract had obvious inhibitory effect on growth of Bel-7402 cells, and the inhibition rate was increased with the extension of incubation time, and the inhibition rate was the highest in the high dose group at 96 hours. The levels of bax gene gray ratio in drug group was significantly higher than that of the blank control group(P<0.01), while bcl-2 gene gray ratio was lower than that of blank control group (P<0.01). Conclusion: The Lycium barbarum water extract can inhibit the proliferation, block cell cycle and induce apoptosis of Bel-7402 liver cancer cell. It can increase ROS level, promote bax gene expression and inhibit bcl-2 gene expression. The results can provide experimental evidence for the anti liver cancer effect of Lycium barbarum. |
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