| 葛 睿,李 盼,周 艳,穆 欣,张 键,韩 丹,张子茜,牟宽厚.奥拉帕尼对黑素瘤细胞的作用及机制研究[J].,2019,19(20):3837-3840 |
| 奥拉帕尼对黑素瘤细胞的作用及机制研究 |
| The Effect of Olaparib on Melanoma Cells and Its Mechanism |
| 投稿时间：2019-02-28 修订日期：2019-03-23 |
| DOI：10.13241/j.cnki.pmb.2019.20.008 |
| 中文关键词: 黑素瘤 奥拉帕尼 凋亡 细胞周期 |
| 英文关键词: Melanoma Olaparib Apoptosis Cell cycle |
| 基金项目:国家自然科学基金项目（81802727）；西安交通大学第一附属医院院基金项目(2016QN-07)；中央高校基本业务费暨西安交通大学校基金（xzy012019098） |
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| 中文摘要: |
| 摘要 目的：探讨奥拉帕尼对黑素瘤细胞的作用及其机制。方法：应用不同浓度的奥拉帕尼处理黑素瘤细胞，利用CCK8检测肿瘤细胞活性。应用Western blot技术检测奥拉帕尼处理黑素瘤细胞后肿瘤细胞内凋亡及周期相关蛋白表达水平。结果：与对照组相比，5 μM奥拉帕尼处理黑素瘤A2058细胞即可抑制肿瘤细胞活性（85.53±2.593）%。随着奥拉帕尼处理浓度倍增对黑素瘤细胞活性的抑制作用越强。在10 μM、20 μM、40 μM、80 μM奥拉帕尼处理浓度下，黑素瘤细胞活性分别是（68.88±1.484）%、（47.21±1.759）%、（33.04±1.261）%、（28.17±1.731）%。奥拉帕尼处理黑素瘤细胞后可促进肿瘤细胞内凋亡相关蛋白PARP1剪切体表达增加，并可抑制细胞周期相关蛋白cyclin D1的表达。结论：奥拉帕尼通过促进黑素瘤细胞凋亡及抑制肿瘤细胞周期蛋白表达的机制发挥抑制黑素瘤细胞活性的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of olaparib on melanoma cells and its mechanism. Methods: CCK8 was used to detect the viability of melanoma cells after being treated with olaparib at different concentrations. Western blot was used to detect expression of proteins related with apoptosis and cell cycle in melanoma cells treated with olaparib. Results: Compared with the control group, 5 μM olaparib can inhibit the viability of melanoma cells (85.53±2.593)%. And the inhibitory effect of olaparib on the viability of melanoma cells was stronger as its concentration doubling. The viability of melanoma cells were changed to (68.88±1.484)%, (47.21±1.759)%, (33.04±1.261)%, (28.17±1.731)% after the treatment of olaparib at 10 μM, 20 μM, 40 μM, 80 μM concentrations respectively. Olaparib treatment can promote the expression of apoptosis-related cleaved-PARP1 and inhibit the expression of cell cycle-related cyclin D1 in melanoma cells. Conclusion: Olaparib play a role in inhibiting the viability of melanoma cells by promoting the apoptosis and inhibiting the expression of cell cycle protein. |
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