| 梁 衡,于宏珠,毛 昀,路晓光,谢 青,唐劲天,李利亚.中频交变微电流联合紫杉醇抗A549细胞作用的研究[J].,2020,(1):24-29 |
| 中频交变微电流联合紫杉醇抗A549细胞作用的研究 |
| Effect of Alternating Micro-current at Intermediate Frequency(ACIF) Combined with Paclitaxel on A549 Cells |
| 投稿时间：2019-05-21 修订日期：2019-06-18 |
| DOI：10.13241/j.cnki.pmb.2020.01.005 |
| 中文关键词: 中频交变微电流 紫杉醇注射液 A549细胞 细胞周期 凋亡 |
| 英文关键词: Alternating Micro-curren at Intermediate Frequency(ACIF) Paclitaxel injection A549 cells Cell cycle Apoptosis |
| 基金项目:国家自然科学基金项目（81372412） |
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| 中文摘要: |
| 摘要 目的：研究中频交变微电流联合紫杉醇注射液抗A549细胞的作用及机制。方法：对处于对数生长期的肺腺癌A549细胞施加电刺激、紫杉醇及电刺激联合紫杉醇三种不同处理，采用MTT法检测A549细胞存活率，并利用流式细胞仪测量分析各组细胞凋亡/死亡比例及细胞周期状态。结果：通过不同参数的中频交变微电流刺激A549细胞，得到的最低细胞存活率（参数150 kHz、90 mA、30 min）为78.02±0.73%（P<0.01）；联合紫杉醇注射液半抑制浓度（IC50）干预后，细胞存活率为32.87±0.94%（P<0.01）；同时发现中频交变微电流联合紫杉醇注射液能促进A549细胞凋亡，阻滞细胞于S期、G2/M期。结论：中频交变微电流可抑制A549细胞增殖、促进凋亡，但对细胞周期影响不明显；与紫杉醇注射液联合应用时具有协同增强抗肿瘤作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect and mechanism of Alternating Micro-current at Intermediate Frequency(ACIF) in combination with paclitaxel injection against A549 cells in vitro. Methods: The A549 cells was exposed to ACIF, paclitaxel and the combination of both in logarithmic phase. The cell growth rate was measured by MTT assay. Cell Apoptosis/death rate and cell cycle status were analyzed by flow cytometry. Results: A549 cells were exposed to different ACIF parameters. The lowest cell survival rate was 78.02±0.73% (parameter 150 kHz, 90 mA, 30 min) (P<0.01). After the intervention of paclitaxel (IC50), the cell survival rate was 32.87± 0.94%(P<0.01). It was found that the combination of ACIF and paclitaxel can promote apoptosis of A549 cells, and block cells in S and G2/M phases. Conclusion: ACIF can promote apoptosis of A549 cells, but has no obvious effect on cell cycle. When combined with paclitaxel injection, it has synergistic enhancement of anti-tumor effect. |
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