| 张雪华,刘雪环,孙向荣,金婷婷,徐 珞.DVC orexin-A通过调控ghrelin受体信号通路改善顺铂所致大鼠胃功能紊乱[J].,2020,(4):643-647 |
| DVC orexin-A通过调控ghrelin受体信号通路改善顺铂所致大鼠胃功能紊乱 |
| Effect of DVC Orexin-A on the Gastric Dysfunction Induced by Cisplatin Via Regulating the Ghrelin Receptor Signaling Pathway |
| 投稿时间：2019-05-23 修订日期：2019-06-18 |
| DOI：10.13241/j.cnki.pmb.2020.04.009 |
| 中文关键词: Orexin-A DVC 顺铂 胃功能紊乱 |
| 英文关键词: Orexin-A DVC Cisplatin Gastric dysfunction |
| 基金项目:国家自然科学基金项目（81270460） |
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| 中文摘要: |
| 摘要 目的：探讨迷走神经复合体（Dorsal vagal complex，DVC）内orexin-A对顺铂所致胃功能紊乱的影响及可能机制。方法：随机选取30只大鼠并将其分为3组（n=10）：对照组（NS组）；24 h顺铂治疗组；48 h顺铂治疗组。24 h顺铂治疗组和48 h顺铂治疗组大鼠分别在腹腔注射顺铂后24 h和48 h处死大鼠，对照组大鼠腹腔注射生理盐水（Normal saline，NS）。采用定量实时PCR检测各组大鼠下丘脑orexin-A mRNA的表达，ELISA测量大鼠脑脊液中P物质水平；DVC内微量注射orexin-A和ghrelin受体拮抗剂后，检测大鼠食物和高岭土的摄入量。结果：顺铂可显著减少大鼠下丘脑orexin-A mRNA表达，增加其脑脊液内P物质的浓度。外源性orexin-A可改善顺铂引起的厌食症和异食癖。orexin-A以上的效应可被DVC内预先注射ghrelin受体拮抗剂部分逆转。结论：orexin-A可能通过ghrelin神经肽系统改善顺铂在化疗过程中诱导的胃功能紊乱。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of orexin-A in Vagus nerve complex (DVC) on cisplatin-induced gastric dysfunction and its possible mechanism. Methods: Thirty rats were randomly selected and divided into 3 groups (n=10): control group (NS group); 24 h cisplatin-treated group; 48 hcisplatin-treated group. Rats in the 24 h cisplatin-treated group and the 48h cisplatin-treated group were sacrificed 24 h and 48 h after intraperitoneal injection of cisplatin. Rats in the control group were intraperitoneally injected with normal saline(NS). Quantitative real-time PCR was used to measure the expression of orexin-A mRNA in hypothalamus of each group of rats. The concentration of substance P in cerebrospinal fluid of rats was measured by ELISA; After microinjection of orexin-A and ghrelin receptor antagonists into DVC, the intake of rat food and kaolin was measured in cisplatin treated rats. Results: Cisplatin significantly reduced orexin-A mRNA expression in the hypothalamus, and cisplatin increases the concentration of substance P in the cerebrospinal fluid of rats. Exogenous orexin-A improve cisplatin-induced anorexia and pica. The effects of above orexin-A can be partially blocked by pre-injection of ghrelin receptor antagonists in DVC. Conclusion: Orexin-A may improve the gastric dysfunction induced by cisplatin during chemotherapy through the ghrelin neuropeptide system. |
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