| 唐友红,孙 彦,贺 欣,余 喆,吴 红.基于聚苹果酸的贻贝仿生粘合剂的制备及性能研究[J].,2020,(5):818-821 |
| 基于聚苹果酸的贻贝仿生粘合剂的制备及性能研究 |
| Preparation and Properties of Mussel-Inspired Adhesive Based on Poly (β-malic acid) |
| 投稿时间：2019-10-06 修订日期：2019-10-30 |
| DOI：10.13241/j.cnki.pmb.2020.05.004 |
| 中文关键词: 聚苹果酸 多巴胺 贻贝仿生 粘合剂 |
| 英文关键词: Poly (β-malic acid) Dopamine Mussel-inspired Adhesive |
| 基金项目:国家自然科学基金项目（81571786；31771087） |
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| 中文摘要: |
| 摘要 目的：以实验室制得的聚苹果酸(PMLA)为高分子骨架，通过酰胺化反应将多巴胺(DA)分子连接到聚苹果酸，制得既具有良好粘合性能，又有优异生物相容性的贻贝仿生粘合剂。方法：L-天冬氨酸通过内酯开环聚合法合成高分子化合物聚苹果酸，将PMLA与DA加EDC/NHS反应得到粘合剂PMLA-DA，傅里叶变换红外光谱、核磁氢谱、紫外可见光光谱等对其进行结构表征，标准曲线法测多巴胺取代度，采用搭接剪切测试法评估粘合剂对不锈钢、玻璃、猪皮三种材质的粘合强度，MTT法检测贻贝仿生粘合剂的细胞毒性，通过降解试验验证PMLA-DA的降解性能。结果：测得PMLA-DA中多巴胺的取代度能达到21.3 %，搭接剪切试验测得粘合剂对猪皮的粘合强度为22.68 kPa，高于目前市场上常用的生物医用粘合剂纤维蛋白胶15.38 kPa的粘合强度，PMLA-DA对不锈钢与玻璃亦有很好的粘合性能。细胞毒性研究和体外降解试验显示PMLA-DA无细胞毒性，降解性能良好。结论：通过聚苹果酸与多巴胺反应制得贻贝仿生粘合剂PMLA-DA，该粘合剂对多种材质均具有良好的粘合力；无细胞毒性，降解性能良好，对皮肤组织的粘合强度优于目前商用的生物粘合剂纤维蛋白胶。无论是对无机材料间的粘接，或者是医学领域的伤口粘合，均具有良好的应用前景。 |
| 英文摘要: |
| ABSTRACT Objective: Using the laboratory made poly (β-malic acid) (PMLA) as a polymer backbone to attach dopamine (DA) by amidation reaction, obtain a mussel-inspired adhesive with good adhesion and excellent biocompatibility. Methods: The polymer compound PMLA was prepared from L-aspartic acid by lactone ring-opening polymerization method, then PMLA and DA react with EDC/NHS to get the adhesive PMLA-DA. It is characterized by FT-IR, 1H-NMR and UV-Vis. Determination of dopamine substitution degree by standard curve method. The adhesion strength of PMLA-DA to stainless steel, glass and porcine skin are evaluated by lap shear strength test. The cytotoxicity of the mussel-inspired adhesive is detected by MTT experiment. Using in-vitro experiment to verify the degradation performance of PMLA-DA. Results: The dopamine substitution degree in PMLA-DA could reach 21.3%, and the adhesion strength to porcine skin was 22.68 kPa, which was higher than the clinically used fibrin glue's 15.38 kPa, PMLA-DA also has good adhesion to stainless steel and glass. Cytotoxicity experiments and in vitro degradation tests show that PMLA-DA is non-cytotoxic and has good degradation performance. Conclusion: A mussel-inspired adhesive PMLA-DA was prepared from poly (β-malic acid) and dopamine, this adhesive has good adhesion to a variety of materials with no cytotoxicity, and good degradation performance.The adhesion strength to skin tissue is superior to the current commercial bio-adhesive fibrin glue. Its biocompatibility is better than cyanoacrylate adhesive. Whether it is bonding between inorganic materials or wound adhesion in the medical field, it has a good application prospect. |
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