| 郭 宇,陈 伟,殷祥瑞,杨兴智,徐 斌,熊 伟.尿源干细胞对神经源性膀胱大鼠膀胱功能及Notch1、Jagged1蛋白表达的影响[J].,2020,(6):1047-1050 |
| 尿源干细胞对神经源性膀胱大鼠膀胱功能及Notch1、Jagged1蛋白表达的影响 |
| Effects of Urine Stem Cells on Bladder Function and Expression of Notch1 and Jagged1 Proteins in Neurogenic Bladder Rats |
| 投稿时间：2019-10-06 修订日期：2019-10-28 |
| DOI：10.13241/j.cnki.pmb.2020.06.010 |
| 中文关键词: 神经源性膀胱 膀胱功能 尿源干细胞 Notch1 Jagged1 |
| 英文关键词: Neurogenic bladder Bladder function Urine stem cells Notch1 Jagged1 |
| 基金项目:重庆市卫生计生委医学科研项目（2016MSXM074） |
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| 中文摘要: |
| 摘要 目的：研究尿源干细胞对神经源性膀胱大鼠膀胱功能及Notch1、Jagged1蛋白表达的影响。方法：纳入60只健康雌性清洁SD大鼠作为实验对象，将其按照随机抽签法分为正常对照组、研究组以及损伤组，每组各20只。其中研究组和损伤组大鼠均建立神经源性膀胱模型，研究组在造模成功后予以尿源性干细胞尾静脉注射。28 d后，比较三组大鼠膀胱功能相关指标水平，膀胱湿质量和膀胱湿质量/体质量，Notch1、Jagged1蛋白表达水平。结果：损伤组收缩时间、排尿量、膀胱峰压均低于正常对照组，而研究组收缩时间、排尿量、膀胱峰压均高于损伤组（均P＜0.05）；损伤组膀胱基压高于正常对照组，而研究组膀胱基压低于损伤组（均P＜0.05）。损伤组膀胱湿质量和膀胱湿质量/体质量均高于正常对照组，而研究组膀胱湿质量和膀胱湿质量/体质量低于损伤组（均P＜0.05）。损伤组Notch1、Jagged1蛋白表达水平均高于正常对照组，而研究组Notch1、Jagged1蛋白表达水平低于损伤组（均P＜0.05）。结论：尿源干细胞的应用可显著改善神经源性膀胱大鼠膀胱功能，同时可下调Notch1、Jagged1蛋白表达水平。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of urine stem cells on bladder function and expression of Notch1 and Jagged1 proteins in neurogenic bladder rats. Methods: 60 healthy female clean SD rats were included as the study subjects, and they were divided into normal control group, study group and injury group by random lottery, with 20 rats in each group. Neurogenic bladder models were established in the study group and the injury group, and urine stem cells were injected into the tail vein after successful modeling in the study group. After 28 days, the levels of indicators related to bladder function, bladder wet mass and bladder wet mass/body mass, protein expression levels of Notch1 and Jagged1 in the three groups of rats were compared. Results: The contraction time, urine output and bladder peak pressure in the injury group were lower than those in the normal control group, while the contraction time, urine output and bladder peak pressure in the study group were higher than those in the injury group (all P＜0.05). The bladder basal pressure in the injury group was higher than that in the normal control group, while the bladder basal pressure in the study group was lower than that in the injury group (all P＜0.05). The wet bladder mass and wet bladder mass/body mass in the injury group were higher than those in the normal control group, while the wet bladder mass and wet bladder mass/body mass in the study group were lower than those in the injury group (all P＜0.05). The protein expression levels of Notch1 and Jagged1 in the injury group were higher than those in the normal control group, while the protein expression levels of Notch1 and Jagged1 in the study group were lower than those in the injury group (all P＜0.05). Conclusion: The application of urine stem cells can significantly improve the bladder function of neurogenic bladder rats, and it can down-regulate the protein expression levels of Notch1 and Jagged1. |
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