| 王 科,王应强,叶 静,张 雪,钟 玲,杨 力,马世丽.LncRNA RP11-316M1.12在甲状腺乳头状癌中的表达及对细胞侵袭、迁移的影响[J].,2020,(14):2646-2650 |
| LncRNA RP11-316M1.12在甲状腺乳头状癌中的表达及对细胞侵袭、迁移的影响 |
| Expression of LncRNA RP11-316M1.12 in Papillary Thyroid Carcinoma and Its Effects on Cell Invasion and Migration |
| 投稿时间：2020-03-23 修订日期：2020-04-18 |
| DOI：10.13241/j.cnki.pmb.2020.14.009 |
| 中文关键词: 长链非编码RNA RP11-316M1.12 甲状腺乳头状癌 增殖 侵袭 迁移 |
| 英文关键词: Long non-coding RNA RP11-316M1.12 Papillary thyroid carcinoma Proliferation Invasion Migration |
| 基金项目:四川省卫计委基金项目（19PJ141） |
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| 中文摘要: |
| 摘要 目的：探讨长链非编码RNA（LncRNA）RP11-316M1.12在甲状腺乳头状癌（PTC）中的表达及对细胞侵袭、迁移的影响。方法：采用荧光定量PCR（qRT-PCR）法检测42例PTC组织及其相应癌旁组织中LncRNA RP11-316M1.12表达水平。体外培养TPC-1细胞，将TPC-1细胞分为LncRNA RP11-316M1.12-siRNA组（敲低组）、阴性对照组（NC组）、空白对照组（NG组），qRT-PCR法检测各组TPC-1细胞中LncRNA RP11-316M1.12表达水平，CCK-8法检测各组TPC-1细胞增殖能力，Transwell实验检测各组TPC-1细胞迁移、侵袭能力，Western blot法检测各组TPC-1细胞中上皮-间充质转化（EMT）相关蛋白表达水平。结果：与癌旁组织相比，PTC癌组织中LncRNA RP11-316M1.12相对表达量明显升高（P<0.05）。与NC组、NG组比较，转染24、48、72 h敲低组TPC-1细胞增殖能力受到抑制（P<0.05）。与NC组、NG组比较，敲低组迁移细胞数、侵袭细胞数、间质细胞标志物波形蛋白（vimentin）、N-钙粘附蛋白（N-cadherin）蛋白相对表达量均显著降低（P<0.05），上皮细胞标志物E-钙粘附蛋白（E-cadherin）相对表达量显著升高（P＜0.05）。结论：LncRNA RP11-316M1.12在PTC癌组织中呈高表达，沉默LncRNA RP11-316M1.12可抑制TPC-1细胞增殖、迁移、侵袭能力，其机制可能与PTC肿瘤细胞EMT过程有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of long non-coding RNA (LncRNA) RP11-316M1.12 in papillary thyroid carcinoma (PTC) and its effects on cell invasion and migration. Methods: The expression levels of LncRNA RP11-316M1.12 in PTC tissues and its corresponding paracancerous tissues of 42 cases were detected by fluorescence quantitative PCR (qRT-PCR). TPC-1 cells were cultured in vitro and divided into LncRNA RP11-316M1.12-siRNA group (Knockdown group), negative control group (NC group), blank control group (NG group), the expression of LncRNA RP11-316M1.12 in each group of TPC-1 cells was detected by qRT-PCR, CCK-8 method was used to detect the proliferation of each group of TPC-1 cells, the cell migration and invasion of each group of TPC-1 cells was detected by Transwell test, Western blot method was used to detect the expression levels of epithelial-mesenchymal transition (EMT) related proteins in each group of TPC-1 cells. Results: Compared with the paracancerous tissues, the relative expression of LncRNA RP11-316M1.12 was significantly higher in PTC tissues(P<0.05). Compared with NC group and NG group, the proliferation of TPC-1 cells was inhibited in knockdown group after transfection for 24, 48, 72 h(P<0.05). Compared with NC group and NG group, the number of migrating cells, invasive cells, the relative expressions of stromal cell markers vimentin(vimentin) and N-epithelial cadherin (N-cadherin) decreased significantly(P<0.05), the relative expression of epithelial cell marker E-epithelial cadherin(E-cadherin) increased significantly (P<0.05). Conclusion: LncRNA RP11-316M1.12 is highly expressed in PTC tissues, silent LncRNA RP11-316M1.12 can inhibit the proliferation, migration and invasion of TPC-1 cells and its mechanism may be related to the EMT processes of PTC tumor cells. |
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