| 沈培根,卢秋燕,王丽惠,卢燕辉,陈碧芬.谷氨酰胺联合恩替卡韦对病毒型肝硬化小鼠肝脏病变指标及免疫水平的影响[J].,2020,(14):2651-2655 |
| 谷氨酰胺联合恩替卡韦对病毒型肝硬化小鼠肝脏病变指标及免疫水平的影响 |
| Effects of Glutamine Combined with Entecavir on Liver Lesion Index and Immune Level in Virus-type Cirrhosis Mice |
| 投稿时间：2020-02-27 修订日期：2020-03-23 |
| DOI：10.13241/j.cnki.pmb.2020.14.010 |
| 中文关键词: 病毒型肝硬化 小鼠 谷氨酰胺 恩替卡韦 |
| 英文关键词: Virus-type cirrhosis Mice Glutamine Entecavir |
| 基金项目:福建省自然科学基金项目(2016J01575) |
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| 中文摘要: |
| 摘要 目的：探讨谷氨酰胺联合恩替卡韦对病毒型肝硬化小鼠肝脏病变指标及免疫水平的影响。方法：将 60只健康雄性BABL/cJ 小鼠按照随机数字表法分为模型组、谷氨酰胺组，谷氨酰胺联合恩替卡韦组（联合组），对照组，每组15只。采用高压水注射方法将50 μL HBV质粒通过尾静脉注入模型组、谷氨酰胺组，联合组三组小鼠体内,然后腹腔注射50%CCI4(5 mL/kg)以构建肝硬化模型小鼠。对照组经尾静脉注射 50 μL生理盐水，然后腹腔注射生理盐水 (5 mL/kg)。谷氨酰胺组接受谷氨酰胺（0.5 mL/10g）治疗，联合组接受恩替卡韦联合谷氨酰胺（0.5 mL/10 g）治疗，对照组、模型组接受生理盐水（0.5 mL/10g）治疗，每天1次，治疗周期均为4周。比较四组小鼠的肝功能、病毒载量、肝纤维化指标、免疫功能。结果：成模后，模型组、谷氨酰胺组、联合组的病毒载量、ALT、AST、HA、PIIIP水平无统计学差异（P＞0.05）；治疗4周后，谷氨酰胺组、联合组两组病毒载量、ALT、AST、HA、PIIIP水平显著下降，且联合组病毒载量、ALT、AST、HA、PIIIP水平低于谷氨酰胺组，差异具有统计学差异（P<0.05）；治疗4周后，谷氨酰胺组病毒载量、ALT、AST、HA、PIIIP水平低于模型组，差异具有统计学差异（P<0.05）；治疗4周后，谷氨酰胺组、联合组两组脾脏指数、CD4+、CD4+/CD8+百分率升高组脾脏指数、CD4+、CD4+/CD8+百分率高于谷氨酰胺组，差异具有统计学差异（P<0.05）。结论：谷氨酰胺联合恩替卡韦可改善病毒型肝硬化小鼠肝脏肝功能，减轻组织纤维化，并有助于增强其免疫功能。 |
| 英文摘要: |
| ABSTRACT Objective: To explore effects of glutamine combined with entecavir on liver lesion index and immune level in virus-type cirrhosis mice. Methods: Sixty healthy male BABL/cJ mice were divided into model group, glutamine group, glutamine combined with entecavir group (combined group) and control group according to the random number table method，15 mice in each group. Mice of the model group, glutamine group and the combined group were injected with 50 μL HBV plasmids through the tail vein by high-pressure water injection, and then 50%CCI4(5 mL/kg) was intraperitoneally injected into the mice of the cirrhosis model. In the control group, 50 μL of normal saline was injected via tail vein, followed by intraperitoneal injection of normal saline (5 mL/kg).The glutamine group received glutamine (0.5 mL/10g), the combined group received entecavir combined with glutamine (0.5 mL/10g), the control group and the model group received normal saline (0.5 mL/10g), once a day, the treatment cycle was 4 weeks.The liver function, viral load, liver fibrosis index and immune function of four groups of mice were compared. Results: After modeling, there was no statistical difference in the levels of virus load, ALT, AST, HA and PIIIP in the model group, the glutamine group and the combined group (P>0.05). After 4 weeks of treatment, the levels of viral load, ALT, AST, HA and PIIIP in the glutamine group and the combined group decreased significantly, and the levels of viral load, ALT, AST, HA and PIIIP in the combined group were lower than those in the glutamine group, with statistically significant differences (P<0.05). After 4 weeks of treatment, the levels of viral load, ALT, AST, HA and PIIIP in the glutamine group were lower than those in the model group, with statistically significant differences (P<0.05). After 4 weeks of treatment, the levels of viral load, ALT, AST, HA and PIIIP in the glutamine group were lower than those in the model group, with statistically significant differences (P<0.05). After 4 weeks of treatment, the spleen index and the percentage of CD4+, CD4+/CD8+ of the glutamine group and the combined group increased, and the spleen index and the percentage of, CD4+, CD4+/CD8+ of the combined group were higher than that of the glutamine group, with statistically significant differences(P<0.05). Conclusion: Glutamine combined with entecavir can improve the liver function of virus-type cirrhosis mice, reduce tissue fibrosis and increase their immune function. |
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