| 赵 晶,林 江,宋铁砾,代 冬,刘 冰.纤维调节素抑制脂多糖诱导性动物牙周炎的实验分析[J].,2021,(7):1208-1211 |
| 纤维调节素抑制脂多糖诱导性动物牙周炎的实验分析 |
| Experimental Analysis of Fibronectin Inhibiting Lipopolysaccharide-induced Periodontitis in Animals |
| 投稿时间：2020-10-03 修订日期：2020-10-27 |
| DOI：10.13241/j.cnki.pmb.2021.07.002 |
| 中文关键词: 纤维调节素 脂多糖 大鼠 牙周炎 蛋白酪氨酸磷酸酶-2 |
| 英文关键词: Fibromodulin Lipopolysaccharide Rats Periodontitis Protein Tyrosine Phosphatase-2 |
| 基金项目:首都医学发展科研基金项目（2016-1-4051） |
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| 中文摘要: |
| 摘要 目的：探讨与分析纤维调节素抑制脂多糖诱导性大鼠牙周炎的效果与机制。方法：将健康雌性Wistar大鼠36只平分为三组-对照组、牙周炎组与纤维调节素组。对照组给予正常喂养，不给予任何干预；牙周炎组与纤维调节素组都给予建立脂多糖诱导性牙周炎模型，建模后2 w纤维调节素组每天给予纤维调节素80 mg/kg?d，牙周炎组给予等体积的生理盐水灌胃治疗，持续4 w。结果：牙周炎组与纤维调节素组治疗第2 w与第4 w的牙龈指数、探诊深度、牙槽骨吸收值高于对照组(P<0.05)，纤维调节素组低于牙周炎组(P<0.05)。牙周炎组与纤维调节素组治疗第2 w与第4 w的血清骨钙素(osteocalcin，OCN)值高于对照组(P<0.05)，碱性磷酸酶(Alkaline phosphatase，ALP)值低于对照组(P<0.05)，牙周炎组与纤维调节素组对比差异也都有统计学意义(P<0.05)。牙周炎组与纤维调节素组治疗第2 w与第4 w的蛋白酪氨酸磷酸酶-2(Src Homology Phosphotyrosyl Phosphatase 2，SHP-2)含蛋白相对表达水平高于对照组(P<0.05)，纤维调节素组低于牙周炎组(P<0.05)。结论：纤维调节素可抑制脂多糖诱导性大鼠牙周炎的进展，抑制OCN与SHP-2蛋白的表达，促进ALP的表达，从而改善牙周炎大鼠的相关症状。 |
| 英文摘要: |
| ABSTRACT Objective: To explore and analysis the effect and mechanism of fibronectin on inhibiting lipopolysaccharide-induced periodontitis in rats. Methods: A total of 36 healthy female Wistar rats were equally divided into three groups-control group, periodontitis group and fibronectin group. The control group was given normal feeding without any intervention; the fibronectin group was given 80 mg/kg?d of fibronectin every day for 2 weeks, and the periodontitis group was given an equal volume of normal saline gavage treatment for 4 weeks. Results: At the 2nd and 4th week of treatment, the gingival index, probing depth, and alveolar bone resorption values of the periodontitis group and the fibronectin group were higher than those of the control group (P<0.05), and the fibronectin group was lower than the periodontitis group (P<0.05); the serum osteocalcin (OCN) values of the periodontitis group and fibronectin group were higher than those of the control group (P<0.05), and alkaline phosphatase (ALP) values were lower than those of the control group (P<0.05), there were statistically significant differences between the periodontitis group and the fibronectin group (P<0.05); the relative expression level of Src Homology Phosphotyrosyl Phosphatase 2(SHP-2) of the periodontitis group and the fibronectin group were higher than that of the control group (P<0.05), the fibronectin group was lower than the periodontitis group (P<0.05). Conclusion: Fibromodulin can inhibit the progression of lipopolysaccharide-induced periodontitis in rats, inhibit the expression of OCN and SHP-2 protein, and promote the expression of ALP, thereby improving the related symptoms of periodontitis rats. |
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