文章摘要
王钺洋,王世远,李健瑛,王 成,姜辰一,赵福军.长链非编码RNA LINC01006对前列腺癌细胞增殖和侵袭的影响[J].,2021,(8):1401-1407
长链非编码RNA LINC01006对前列腺癌细胞增殖和侵袭的影响
Influence of Long Non-coding RNA LINC01006 on the Proliferation and Invasion of Prostate Cancer Cell
投稿时间:2020-10-07  修订日期:2020-10-30
DOI:10.13241/j.cnki.pmb.2021.08.001
中文关键词: 前列腺癌  lncRNA  细胞增殖  细胞侵袭  转录因子
英文关键词: Prostate cancer  LncRNA  Cell proliferation  Cell invasion  Transcription factor
基金项目:国家自然科学基金项目(81772746)
作者单位E-mail
王钺洋 上海交通大学附属第一人民医院泌尿外科 上海交通大学医学院 上海 200080 Urowangyy@163.com 
王世远 上海交通大学附属第一人民医院泌尿外科 上海交通大学医学院 上海 200080  
李健瑛 上海交通大学附属第一人民医院泌尿外科 上海交通大学医学院 上海 200080  
王 成 江阴市人民医院泌尿外科 江苏 无锡 214400  
姜辰一 上海交通大学附属第一人民医院泌尿外科 上海交通大学医学院 上海 200080  
赵福军 上海交通大学附属第一人民医院泌尿外科 上海交通大学医学院 上海 200080  
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中文摘要:
      摘要 目的:探究长链非编码RNA LINC01006对前列腺癌(prostate cancer, PCa)细胞增殖和侵袭能力的影响。方法:体外培养人前列腺正常上皮细胞系RWPE-1,人PCa细胞系LNCaP、22Rv1、PC3、C4-2b,应用实时定量PCR(qRT-PCR)技术检测上述细胞LINC01006的表达;分别通过转染小干扰RNA(siRNA)或过表达LINC01006的慢病毒载体,在LNCaP和PC3细胞中敲减LINC01006或稳定过表达LINC01006;应用CCK8、克隆形成实验检测LINC01006对PCa细胞增殖能力的影响;应用Transwell侵袭实验检测LINC01006对PCa细胞侵袭能力的影响;通过网站预测LINC01006的转录调控因子及其结合位点。结果:相较于正常前列腺上皮细胞系RWPE-1,PCa细胞系LNCaP、22Rv1、C4-2b和PC3中LINC01006表达明显升高(P<0.05)。敲减LINC01006后的PCa细胞系LNCaP和PC3的增殖和侵袭能力被显著抑制(P<0.05),过表达LINC1006则明显促进PCa细胞系LNCaP和PC3的增殖、侵袭能力(P<0.05)。通过PROMO网站预测可见AR是LINC01006的潜在转录调控因子,通过Cistrome DB数据库发现LINC01006上游启动子区域存在AR富集;敲减、抑制AR后LNCaP细胞中LINC01006表达明显升高(P<0.05)。结论:LINC01006在PCa细胞系中呈高表达,促进PCa细胞的增殖和侵袭,其受到AR负向调控,可能在PCa发生发展和去势抵抗形成过程中发挥作用。
英文摘要:
      ABSTRACT Objective: To explore the effects of long non-coding RNA LINC01006 on the proliferation and invasion of prostate cancer (PCa) cells. Methods: Human prostate normal epithelial cell line RWPE-1 and human PCa cell lines LNCaP, 22Rv1, PC3 and C4-2b were cultured in vitro, and the expression of LINC01006 was detected by real-time quantitative PCR (qRT-PCR). The effect of LINC01006 on the proliferation of PCa cells was detected by CCK8 and colony formation assays. Transwell chamber was used to evaluate the effect of LINC01006 on the invasion ability of PCa cells. Transcriptional regulatory factors and their binding sites of LINC01006 were predicted through the website. Results: Compared with normal prostate epithelial cell line RWPE-1, the expression of LINC01006 in PCa cell lines LNCaP, 22Rv1, C4-2b and PC3 was significantly increased (P<0.05). Knockdown of LINC01006 significantly inhibited the proliferation and invasion of LNCaP and PC3(P<0.05), and overexpressing LINC1006 significantly promoted the proliferation and invasion of PCa cell lines LNCaP and PC3 (P<0.05). AR was predicted to be a potential transcriptional regulator of LINC01006 using the PROMO website, and was significantly recruited to the upstream promoter region of LINC01006 according to the Cistrome DB database. The expression of LINC01006 in LNCaP cell line increased significantly after silencing or inhibiting A(P<0.05). Conclusion: LINC01006 is highly expressed in PCa cell lines, which promotes the proliferation and invasion of PCa cells. It is negatively regulated by AR and may play a role in the occurrence and development of PCa and the formation of castration resistance.
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