文章摘要
孔军伶,曾令娥,高 妍,邵长玲,沙素红.双歧杆菌对母婴分离大鼠成年后肠道敏感性及结肠脑源性神经营养因子表达的影响[J].,2021,(8):1420-1424
双歧杆菌对母婴分离大鼠成年后肠道敏感性及结肠脑源性神经营养因子表达的影响
Effects of Bifidobacterium on Intestinal Sensitivity and Colonic Brain-derived Neurotrophic Factor Expression in Adult Rats
投稿时间:2020-10-12  修订日期:2020-10-31
DOI:10.13241/j.cnki.pmb.2021.08.004
中文关键词: 双歧杆菌  母婴分离大鼠  肠道敏感性  脑源性神经营养因子
英文关键词: Bifidobacterium  Mother infant isolated rat  Intestinal sensitivity  Brain-derived neurotrophic factor
基金项目:北京市教委科技计划重点项目(KZ201810025033)
作者单位E-mail
孔军伶 首都医科大学燕京医学院检验学学系检验实验中心病原与免疫学实验室 北京101300 bjkjl123@163.com 
曾令娥 首都医科大学燕京医学院教学办公室 北京101300  
高 妍 首都医科大学附属北京朝阳医院消化内科 北京100016  
邵长玲 首都医科大学燕京医学院检验学学系检验实验中心病原与免疫学实验室 北京101300  
沙素红 首都医科大学燕京医学院临床医学学系生物医学实验中心形态学实验室 北京101300  
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中文摘要:
      摘要 目的:探讨双歧杆菌对母婴分离大鼠成年后肠道敏感性及结肠脑源性神经营养因子表达的影响。方法:将60只新生期SD大鼠随机分为NS组(正常组)、MS组(模型组)、MS+Bif组(双歧杆菌干预),每组20只大鼠,应用避水应激模型(chronic water avoidance stress,WAS)避水实验构建大鼠应激波形,通过兴盛时期母婴分离建立大鼠的肠道高敏感模型,新生鼠在断奶之后对溶剂组与双歧杆菌组分别进行灌胃干预,取大鼠的新鲜粪便进行选择性培养基平皿技术方法检测大鼠粪便的菌群代表性菌种数量。到大鼠8 w成年之后应用阶梯体积直肠求精扩张对三组大鼠肠道敏感性进行评价。结果:与对照组相比,模型组大鼠的大肠杆菌和类杆菌数量明显增多,且通过双歧杆菌干预之后大肠杆菌和类杆菌数量下降,三组大鼠大肠杆菌和类杆菌数量差异显著(P<0.05),三组大鼠的双歧杆菌和乳酸菌对比无明显差异(P>0.05);对比大鼠成年时利用阶梯体积为0 mL、0.4 mL、0.8 mL和1.2 mL直肠球囊扩张评价三组大鼠的肠道敏感性发现,NS组与MS组在0 mL和0.4 mL体积球囊出现扩张的时候血管运动反应性(vascular motor reactivity,VMR)对比无明显差异(P>0.05),从0.4 mL到1.2 mL两组大鼠显著提高,且对比出现显著差异(P<0.05),MS组与MS+Bif组,在0 mL到0.4 mL体积球囊出现扩张的时候VMR对比无明显差异(P>0.05),在0.4 mL到1.2 mL之后VMR差异显著(P<0.05);对比大鼠血清中的促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)和促肾上腺皮质激素释放因子(corticotropin releasing factor,CRF)发现,三组ACTH和CRF表达差异显著(P<0.05);对比大鼠结肠中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)发现,三组大鼠的BDNF表达与SP(P物质)表达对比出现显著差异(P<0.05)。结论:母婴分离大鼠成年之后会出现肠道高敏感性现象,应用双歧杆菌干预之后能够调节肠道菌群,稳定结肠脑源性神经因子表达,改善肠道敏感性现象。
英文摘要:
      ABSTRACT Objective: To investigate the effects of Bifidobacterium on intestinal sensitivity and expression of brain-derived neurotrophic factor (BDNF) in adult rats. Methods: A total of 60 neonatal SD rats were randomly divided into NS group (normal group,n=20), MS group (model group, n=20) and MS + BIF group (Bifidobacterium intervention, n=20). The number of representative bacteria in rat feces was detected by selective culture medium plate technique. After the rats reached adult age at 8 weeks, the intestinal sensitivity of the three groups was evaluated by stepwise volume rectocentesis. Results: Compared with the control group, the number of Escherichia coli and bacteroides in the model group was significantly increased, and the number of Escherichia coli and Bacteroides decreased after the intervention of Bifidobacterium. The number of E.coli and bacteroides in the three groups of mice was significantly different (P<0.05), but there was no significant difference in the Bifidobacterium and Lactobacillus among the three groups (P>0.05). The step volume of adult rats was as follows: 0 mL, 0.4 mL, 0.8 mL and 1.2 mL rectal balloon dilation, which was used to evaluate the intestinal sensitivity of rats in the three groups. The VMR of NS group and MS group had no significant difference when 0 mL and 0.4 mL balloon dilation appeared(P>0.05), but the VMR was significantly increased from 0.4 mL to 1.2 mL(P<0.05). There was no significant difference in VMR between MS group and MS+BIF group(P>0.05), but there was significant difference in VMR after 0.4 mL to 1.2 mL(P<0.05). There were significant differences in the expression of ACTH and CRF and in the expression of BDNF and SP among the three groups (P<0.05). Conclusion: The intestinal hypersensitivity phenomenon will appear in adult rats after mother infant separation. Bifidobacterium intervention can regulate intestinal flora, stabilize the expression of brain-derived nerve factors in colon and improve intestinal sensitivity.
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