文章摘要
李星辉,胡明利,张碧凤,刘 旺,杨 波.异氟醚麻醉通过导致神经干细胞的丢失降低幼鼠的神经发生和认知发育的实验研究[J].,2021,(8):1438-1441
异氟醚麻醉通过导致神经干细胞的丢失降低幼鼠的神经发生和认知发育的实验研究
Experimental Study of Isoflurane Anesthesia by Causing the Loss of Neural Stem Cells to Reduce Neurogenesis and Cognitive Development in Young Mice
投稿时间:2020-09-30  修订日期:2020-10-24
DOI:10.13241/j.cnki.pmb.2021.08.008
中文关键词: 异氟醚  幼鼠  神经发生  认知功能  糖原合成酶激酶-3β  葡萄糖调节蛋白8
英文关键词: Isoflurane  Young mice  Neurogenesis  Cognitive function  Glycogen synthase kinase-3β  Glucose regulatory protein 8
基金项目:贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2018-008)
作者单位E-mail
李星辉 贵州中医药大学第二附属医院麻醉科 贵州 贵阳550000 li23099301@163.com 
胡明利 贵州中医药大学第二附属医院肝胆科 贵州 贵阳550000  
张碧凤 贵州中医药大学第二附属医院麻醉科 贵州 贵阳550000  
刘 旺 贵州中医药大学第二附属医院麻醉科 贵州 贵阳550000  
杨 波 贵州中医药大学第二附属医院疼痛科 贵州 贵阳550000  
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中文摘要:
      摘要 目的:探讨异氟醚麻醉通过导致神经干细胞的丢失降低幼鼠的神经发生和认知发育。方法:SPF级雄性Sprague Dawley(SD)幼年大鼠(30只)平分为两组-对照组与异氟醚组,对照组吸入30 %氧气,异氟醚组吸入1.5最低肺泡有效浓度(minimum alveolar concentration,MAC)异氟烷,检测两组神经干细胞丢失降低与幼鼠的神经发生、认知发育情况。结果:两组建模后6 h的逃避潜伏期、Longa评分对比差异无统计学意义(P>0.05),异氟醚组建模后12 h、24 h的逃避潜伏期、Longa评分高于对照组(P<0.05)。两组建模后6 h、12 h、24 h的下肢动脉血氧饱和度在组内与组间对比差异都无统计学意义(P>0.05)。异氟醚组建模后14 d的海马组织葡萄糖调节蛋白8(GRP8)、糖原合成酶激酶-3β(GSK-3β)蛋白相对表达水平都低于对照组(P<0.05)。结论:异氟醚麻醉可通过导致神经干细胞的丢失,抑制血清GRP8、GSK-3β的释放,从而影响幼鼠的神经发生和认知发育。
英文摘要:
      ABSTRACT Objective: To explore that isoflurane anesthesia reduce the neurogenesis and cognitive development of young rats by causing the loss of neural stem cells. Methods: SPF male Sprague Dawley (SD) juvenile rats (30 rats) were equally divided into two groups-control group and isoflurane group. The control group were inhaled 30 % oxygen, and the isoflurane group were inhaled 1.5 minimum alveolar effective concentration (MAC) isoflurane, and were to detect the loss of neural stem cells, neurogenesis and cognitive development in the two groups. Results: There were no statistically significant difference in the escape latency and Longa score compared between the two groups at 6 h after modeling(P>0.05). Thehigher escape latency and Longa score at 12 h and 24 h after modeling in the isoflurane group at 12 h and 24 h after modelingwere more than the control group(P<0.05). There were no significant difference in the arterial blood oxygen saturation of the lower extremities compared between the two groups at 6 h, 12 h, 24 h after modeling (P>0.05). The relative expression levels of glucose-regulated protein 8 (GRP8) and glycogen synthase kinase-3β(GSK-3β) in hippocampus 14 days after modeling in the isoflurane group were lower than those in the control group(P<0.05). Conclusion: Isoflurane anesthesia can inhibit the release of serum GRP8 and GSK-3β by causing the loss of neural stem cells, thereby affect the neurogenesis and cognitive development of young mice.
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