文章摘要
张 茜,宋志涛,董 升,王金霞,闫伏雨,孙 浩.非小细胞肺癌患者血浆miR-17和miR-204水平表达的临床意义及其预测价值分析[J].,2021,(8):1460-1464
非小细胞肺癌患者血浆miR-17和miR-204水平表达的临床意义及其预测价值分析
Clinical Significance and Prognostic Value of Plasma miR-17 and miR-204 Expression in Patients with Non-small Cell Lung Cancer
投稿时间:2020-10-02  修订日期:2020-10-24
DOI:10.13241/j.cnki.pmb.2021.08.013
中文关键词: 非小细胞肺癌  miR-17  miR-204  临床病理参数  预后
英文关键词: Non-small cell lung cancer  miR-17  miR-204  Clinicopathological parameters  Prognosis
基金项目:北京市自然科学基金面上项目(7162069)
作者单位E-mail
张 茜 首都医科大学附属北京朝阳医院检验科 北京 100043 nicezhang123@sina.com 
宋志涛 天津市肺癌研究所/天津医科大学总医院核医学科 天津 300052  
董 升 天津市肺癌研究所/天津医科大学总医院核医学科 天津 300052  
王金霞 北京中医药大学东直门医院检验科 北京 100700  
闫伏雨 北京中医药大学东直门医院检验科 北京 100700  
孙 浩 北京中医药大学东直门医院病理科 北京100700  
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中文摘要:
      摘要 目的:检测非小细胞肺癌(NSCLC)患者血浆miR-17和miR-204表达,分析其表达与临床病理参数的关系及其对NSCLC预后的预测价值。方法:选择我院2015年6月至2017年6月期间收治的117例NSCLC患者(观察组)和同期于我院进行体检的100例健康者(对照组)作为研究对象,采用实时荧光定量PCR (qRT-PCR)检测血浆中miR-17和miR-204表达,分析血浆miR-17、 miR-204表达与NSCLC患者临床病理参数的关系。采用受试者工作特征(ROC)曲线分析血浆miR-17、 miR-204对NSCLC患者预后的预测价值。结果:观察组患者血浆miR-17表达高于对照组(P<0.05)、miR-204表达低于对照组(P<0.05),miR-17表达与淋巴结转移、远处转移有关(P<0.05),miR-204表达与TNM分期、远处转移有关(P<0.05)。ROC曲线分析结果显示miR-17、 miR-204预测 NSCLC患者预后的曲线下面积(AUC)分别为0.821、0.836。miR-17、miR-204两指标的联合检测对NSCLC患者预后的预测价值更高:敏感度和特异度分别为89.36%(42/47)、92.86%(65/70)。结论:NSCLC患者血浆miR-17、miR-204均存在异常表达,且与患者临床病理参数有关,可能作为NSCLC患者预后预测的潜在生物学指标。
英文摘要:
      ABSTRACT Objective: To detect the expression of plasma miR-17 and miR-204 in patients with non-small cell lung cancer (NSCLC), analyze the relationship between its expression and clinicopathological parameters and prognosis, and explore the predictive value of the expression of miR-17 and miR-204 in NSCLC prognosis. Methods: 117 NSCLC patients (observation group) and 100 healthy volunteers (control group) from June 2015 to June 2017 were selected as the research objects. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expressions of miR-17 and miR-204 in plasma, and to analyze the relationship between the expressions of miR-17 and miR-204 in plasma and the clinicopathological parameters of NSCLC. Receiver operator characteristics curve(ROC) analysis was used to evaluate the predictive value of plasma miR-17 and miR-204 for NSCLC prognosis. Results: The expression of plasma miR-17 in the observation group was higher than that in the control group (P<0.05), and the expression of miR-204 was lower than that in the control group (P<0.05). The expression of miR-17 was associated with lymph node metastasis and distant metastasis (P<0.05), and the expression of miR-204 was associated with TNM staging and distant metastasis (P<0.05). ROC analysis showed that the area under the curve (AUC) of miR-17 , miR-204 predict the prognosis of NSCLC was 0.821, 0.836 respectively. The combined application of miR-17 and miR-204 had a higher predictive value for NSCLC prognosis: the sensitivity and specificity were 89.36%(42/47), 92.86% (65/70) respectively. Conclusion: There were abnormal expression of miR-17 and miR-204 in plasma of NSCLC patients, which is related to clinicopathological parameters of NSCLC, and can be used as a biological indicator to predict the prognosis of NSCLC.
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