| 左 静,冯云霞,李光明,原 娜,刘丽娟.利塞膦酸钠对去卵巢大鼠正畸牙齿移动期间破骨细胞中FAK蛋白表达的影响[J].,2022,(2):244-248 |
| 利塞膦酸钠对去卵巢大鼠正畸牙齿移动期间破骨细胞中FAK蛋白表达的影响 |
| Effect of Risedronate Sodium on FAK Protein Expression in Osteoclasts during Orthodontic Rooth Movement in Ovariectomized Rats |
| 投稿时间：2021-09-06 修订日期：2021-09-30 |
| DOI：10.13241/j.cnki.pmb.2022.02.008 |
| 中文关键词: 利塞膦酸钠 去卵巢 正畸牙齿移动 破骨细胞 FAK |
| 英文关键词: Risedronate sodium Ovariectomy Orthodontic tooth movement Osteoclasts FAK |
| 基金项目:山西省卫生健康委科研项目（2019017） |
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| 中文摘要: |
| 摘要 目的：探讨利塞膦酸钠对去卵巢大鼠正畸牙齿移动期间破骨细胞中FAK蛋白表达的影响。方法：将30只雌性大鼠随机分为3组：假手术组、VOX组（卵巢切除+等量注射生理盐水）和利塞膦酸钠治疗组（切除卵巢+每3天腹膜内注射利塞膦酸钠），各10只。通过数字卡尺测量牙齿移动距离。通过蛋白质印迹检测FAK、I型胶原和整合素-β1蛋白表达水平。使用EXA-3000双能X射线BMD测量仪，测量左股骨BMD。通过RT-qPCR检测TRACP、RANKL和BMP-2 mRNA表达水平。结果：第1~3月时，与假手术组相比，VOX组大鼠体重和牙齿移动距离均增加（P<0.05），而与VOX组相比，利塞膦酸钠治疗组大鼠体重和牙齿移动距离均降低（P<0.05）。与假手术组相比，VOX组FAK、I型胶原和整合素-β1蛋白表达水平、tBMD、pBMD、mBMD和dBMD值以及TRACP、RANKL和BMP-2 mRNA水平均显著降低（P<0.05），而与假手术组和VOX组相比，利塞膦酸钠治疗组以上指标均显著增加（P<0.05）。结论：利塞膦酸钠通过调控整合素-β1/FAK信号通路，对去卵巢大鼠的骨吸收、骨质流失和骨强度降低有有效的抑制作用，可以预防和抑制卵巢切除引起的骨质疏松症的作用，这为骨质疏松症的临床治疗提供了新的依据。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of risedronate sodium on FAK protein expression in osteoclasts during orthodontic tooth movement in ovariectomized rats. Methods: 30 female rats were randomly divided into 3 groups: sham operation group, VOX group (oophorectomy+equal injection of saline) and risedronate sodium treatment group (oophorectomy+Risedronate sodium was injected intraperitoneally every three days), 10 each. The tooth movement distance was measured by a digital caliper. The expression levels of FAK, type I collagen and integrin-β1 protein were detected by Western blot. Use EXA-3000 dual-energy X-ray BMD measuring instrument to measure the BMD of the left femur. The expression levels of TRACP, RANKL and BMP-2 mRNA were detected by RT-qPCR. Results: From 1 to 3 months, compared with the sham operation group, the weight and tooth movement distance of rats in the VOX group increased(P<0.05). Compared with the VOX group, the weight and tooth movement distance of the rats in the risedronate sodium treatment group were reduce(P<0.05). Compared with the sham operation group, the VOX group FAK, type I collagen and integrin-β1 protein expression levels, tBMD, pBMD, mBMD and dBMD values, and TRACP, RANKL and BMP-2 mRNA levels were significantly reduced(P<0.05), while compared with the sham operation group and the VOX group, the above indicators in the risedronate sodium treatment group were significantly increased(P<0.05). Conclusion: Risedronate has an effective inhibitory effect on bone resorption, bone loss and bone strength reduction in ovariectomized rats by regulating the integrin-β1/FAK signaling pathway, and can prevent and inhibit the bone quality caused by ovariectomy. The role of osteoporosis, which provides a new basis for the clinical treatment of osteoporosis. |
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