| 秦正巍,王 顺,白 妍,李 元,乔国强,韦 波,付 艺.针刺对PD胃肠功能障碍大鼠不同部位GDNF含量及胃肠生理指标的影响[J].,2022,(4):615-620 |
| 针刺对PD胃肠功能障碍大鼠不同部位GDNF含量及胃肠生理指标的影响 |
| Effect of Acupuncture on GDNF Content and Gastrointestinal Physiological Indexes in Different Parts of PD Gastrointestinal Dysfunction Rats |
| 投稿时间：2021-05-23 修订日期：2021-06-18 |
| DOI：10.13241/j.cnki.pmb.2022.04.004 |
| 中文关键词: 帕金森病 胶质细胞源性神经营养因子(GDNF) 调神畅志针法 |
| 英文关键词: Parkinson's disease Glial cell derived neurotrophic factor (GDNF) Tiaoshen stitch |
| 基金项目:国家自然科学基金项目（81674091） |
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| 中文摘要: |
| 摘要 目的：探讨不同针刺对帕金森（Parkinson's Disease）胃肠功能障碍大鼠中脑黑质、胃、结肠中胶质细胞源性神经营养因子(GDNF)含量变化影响。方法：腹腔注射鱼藤酮溶液建立PD胃肠功能障碍模型并用阿普吗啡（APO）诱导实验检测。将60只已造模大鼠按随机数字表法分成模型组、假手术组、西药组、空白组、调神畅志组和常规针刺共6组，给予相应治疗2周。观察各组大鼠体重变化，检测粪便含水率、胃内容物残留率及小肠推进率，采用免疫组织化学法及ELISA法对黑质、胃、结肠GDNF含量检测。结果：体重和粪便含水率方面：西药组及两针刺组较模型组比，差异显著（P＜0.01），西药组改善程度优于调神组（P＜0.05），调神组改善程度优于常规组（P＜0.05）。小肠推进率和胃内容物残留率：西药组及两针刺组与模型组相比差异明显（P＜0.01）；西药组改善优于调神组（P＜0.05），调神组优于常规组（P＜0.05）。中脑黑质、胃、结肠GDNF变化：空白组及假手术组含量高于模型组（P＜0.01）；西药组及两针刺组较模型组相比，含量升高（P＜0.01），西药组优于调神组（P＜0.05），调神组均优于常规组（P＜0.05）。结论：针刺治疗可提高PD胃肠功能障碍大鼠黑质、胃、结肠中GDNF含量，且调神组优于常规组，与西药组接近。调神畅志针法对PD胃肠功能障碍作用的原理可能是因为提升了结肠、脑、胃的GDNF含量来完成。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of different acupuncture on the content of glial cell derived neurotrophic factor (GDNF) in substantia nigra, stomach and colon of rats with Parkinson's gastrointestinal dysfunction. Methods: The gastrointestinal dysfunction model of PD was established by injecting rotenone solution and detecting with apmorphine induction test. According to random number table method, 60 rats were divided into 6 groups：blank group, sham-operated group, model group, western medicine group, Tiaoshen Changzhi group and ordinary acupuncture group. The rats were treated for 2 weeks. The changes of body weight were observed, fecal moisture content, gastric content residual rate and intestinal propelling rate were detected, and GDNF content in substantia nigra, stomach and colon were detected by immunohistochemistry and ELISA. Results: Body weight and fecal water content: the western medicine group and the two acupuncture groups were significantly increased compared with the model group (P<0.01). The improvement degree in the western medicine group was better than that in the Tiaoshen Changzhi group (P<0.05), and the improvement degree in the Tiaoshen Changzhi group was better than in the ordinary acupuncture group (P<0.05). In terms of intestinal propulsion rate and gastric contents residual rate, there were significant differences between the western medicine group and the two acupuncture groups and model group (P<0.01). The improvement of the western medicine group was better than that of Tiaoshen Changzhi group (P<0.05), the Tiaoshen Changzhi group was better than the ordinary group (P<0.05). The changes of GDNF in substantia nigra, stomach and colon of midbrain were as follows: the contents of GDNF in blank group and sham group were higher than the model group (P<0.01). Compared with the model group, the content of the western medicine group and the two acupuncture groups increased (P<0.01). The efficacy of the western medicine group was better than that of the Tiaoshen Changzhi group (P<0.05), and the Tiaoshen Changzhi group was better than that of the conventional acupuncture group (P<0.05). Conclusion: Acupuncture treatment increased the content of GDNF in substantia nigra, stomach and colon of rats with PD gastrointestinal dysfunction, and the Tiaoshen Changzhi group was better than the conventional group and close to the western medicine group. One of the mechanisms of Tiaoshen Changzhi acupuncture in the treatment of gastrointestinal dysfunction of PD may be due to the enhancement of GDNF in the brain, stomach and colon. |
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