| 虞 琪,丁匀浚毓,高赛楠,姚 微,叶 霖.Dystrophin、MGF及M-Cad在难产大鼠模型盆底肌肉中的表达意义研究[J].,2022,(13):2436-2440 |
| Dystrophin、MGF及M-Cad在难产大鼠模型盆底肌肉中的表达意义研究 |
| Expression of Dystrophin, MGF and M-Cad in Pelvic Floor Muscles of Dystocia Rat Model and Its Significance |
| 投稿时间：2022-01-08 修订日期：2022-01-30 |
| DOI：10.13241/j.cnki.pmb.2022.13.007 |
| 中文关键词: 难产性大鼠 盆底肌肉 压力性尿失禁 肌养蛋白 力生长因子 M型钙调蛋白 |
| 英文关键词: Dystocia rats Pelvic floor muscles Stress urinary incontinence Dystrophin Mechano-growth factor M-Cad |
| 基金项目:江苏省妇幼健康科研项目（F201929） |
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| 中文摘要: |
| 摘要 目的：研究模拟难产大鼠模型盆底肌肉中Dystrophin、MGF及M-Cad的表达，并探讨其表达与压力性尿失禁的相关性。方法：选择未生产过的雌性SD大鼠40只，按照随机数字表法均分为对照组、模型1组、模型2组和模型3组，对照组大鼠正常喂养，模型1组大鼠行阴道扩张手术，模型2组大鼠切除双侧坐骨神经，模型3组大鼠既行阴道扩张术又切除双侧坐骨神经。喂养1月后，称重后处死各组大鼠，并分离盆底肌肉，称重后通过定量PCR法（qPCR）检测Dystrophin、MGF及M-Cad mRNA的表达。结果：与对照组大鼠相比，难产大鼠模型组（模型1组、模型2组和模型3组）体重和盆底肌肉重量无显著变化（P>0.05），漏尿点压力和最大膀胱容量均显著下降（P<0.05），盆底肌肉Dystrophin、MGF及M-Cad mRNA表达均显著下降（P<0.05）。Pearson相关性分析：大鼠盆底肌肉Dystrophin、MGF及M-Cad mRNA表达水平与漏尿点压力均呈密切正相关（Dystrophin：r=0.725，P=0.002；MGF：r=0.702，P=0.018；M-Cad：r=0.838，P<0.001）；鼠盆底肌肉Dystrophin、MGF及M-Cad mRNA表达水平与最大膀胱容量也均呈密切正相关（Dystrophin：r=0.733，P=0.001；MGF：r=0.682，P=0.025；M-Cad：r=0.819，P<0.001）。结论：模拟难产大鼠模型盆底肌肉Dystrophin、MGF及M-Cad的表达降低，并且提示其表达降低可能与压力性尿失禁有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the expression of Dystrophin, MGF and M-Cad in pelvic floor muscles of dystocia rat model, and to explore its relationship with stress urinary incontinence. Methods: Forty unborn female SD rats were selected and divided into 3 groups according to the random number table method: control group, model 1 group, model 2 group and model 3 group. The rats in the control group were fed normally, the rats in the model 1 group underwent guided dilatation surgery, the rats in the model 2 group had bilateral sciatic nerves removed, and the rats in the model 3 group underwent both vaginal dilation and bilateral sciatic nerve removal. After feeding for 1 month, the rats in each group were sacrificed after weighing, and the pelvic floor muscles were separated. After weighing, the expression of Dystrophin, MGF and M-Cad mRNA was detected by quantitative PCR (qPCR). Results: Compared with the control group, the body weight and pelvic floor muscle weight of the dystocia rat model group (model 1, model 2 and model 3) did not change significantly (P>0.05), and the leakage point pressure and maximum bladder volume were both significantly decreased(P<0.05), and the expression of Dystrophin, MGF and M-Cad mRNA in pelvic floor muscles were also both significantly decreased (P<0.05). Pearson correlation analysis: the expression levels of Dystrophin, MGF and M-Cad mRNA in the pelvic floor muscles of rats are closely related to the pressure of the leak point (Dystrophin: r=0.725, P=0.002; MGF: r=0.702, P=0.018 ; M-Cad: r=0.838, P<0.001); the expression levels of Dystrophin, MGF and M-Cad mRNA in rat pelvic floor muscles are also closely correlated with maximum bladder capacity (Dystrophin: r=0.733, P=0.001; MGF: R=0.682, P=0.025; M-Cad: r=0.819, P<0.001). Conclusion: The expression of Dystrophin, MGF and M-Cad in pelvic floor muscles of dystocia rat model decreased, and the decreased expression were thought to be related to stress urinary incontinence. |
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